Buch, Englisch, Band 1867, 276 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 743 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1867, 276 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 743 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8798-6
Verlag: Springer
Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Proteinforschung
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
Weitere Infos & Material
Part 1. Basic Biology and Engineering of ZFPs.- PRDM14, a zinc finger protein, regulates cancer stemness.- Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins.- Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation.- Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology.- Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers.- Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins.- Directed evolution of targeted recombinases for genome engineering.- Optical recording of cellular zinc dynamics with zinc finger based biosensors.- Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins.- Genome editing of MSCs as a platform for cell therapy.- Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity.- Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells.- Selection and characterization of DNA aptamers against FokI nuclease domain.- An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs.- Part 3. Delivery Methods of ZFPs.- Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering.- Gene editing in channel catfish via double electroporation of zinc finger nucleases.- Delivery of mtZFNs into early mouse embryos.- Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain.- In-vivo applications of cell-penetrating zinc finger transcription factors.- Manufacturing and delivering genome-editing proteins.