Liu | Zinc Finger Proteins | Buch | sack.de

Liu Zinc Finger Proteins



Methods and Protocols

1. Auflage 2018, Band: 1867, 276 Seiten, Gebunden, HC runder Rücken kaschiert, Format (B × H): 183 mm x 261 mm, Gewicht: 716 g Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8798-6
Verlag: Springer, Berlin


Liu Zinc Finger Proteins

This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.

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Weitere Infos & Material


Part IChapter 1        PRDM14, a zinc finger protein, regulates cancer stemnessChapter 2     Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteinsChapter 3     Design and application of mA-specific Zinc finger proteins for the readout of DNA methylationChapter 4     Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technologyChapter 5     Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc FingersChapter 6     Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteinsChapter 7        Directed evolution of targeted recombinases for genome engineeringChapter 8     Optical recording of cellular zinc dynamics with zinc finger based biosensorsChapter 9        Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteinsChapter 10      Genome editing of MSCs as a platform for cell therapy Part IIChapter 11   Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cellsChapter 12   Selection and characterization of DNA aptamers against FokI nuclease domainChapter 13   An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs Part IIIChapter 14   Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineeringChapter 15   Gene editing in channel catfish via double electroporation of zinc finger nucleasesChapter 16      Delivery of mtZFNs into early mouse embryosChapter 17   Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brainChapter 18      In-vivo applications of cell-penetrating zinc finger transcription factorsChapter 19      Manufacturing and delivering genome-editing proteins


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