Chloroplast Biotechnology

Methods and Protocols
2. Auflage 2021
ISBN: 978-1-0716-1474-7
Verlag: Springer US

Buch, Englisch, Band 2317, 369 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 721 g

Reihe: Methods in Molecular Biology

This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights of applications in agriculture, industrial biotechnology and healthcare.  

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Maliga, Pal

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Naturwissenschaften Agrarwissenschaften Ackerbaukunde, Pflanzenbau Nutzpflanzen: Biologie, Genetik, Pflanzenzüchtung

Inhaltsverzeichnis

Dedication…Preface…Table of Contents…Contributing Authors…
Part I Background 
1. The Plastid Genomes of Flowering Plants: Essential PrinciplesTracey A. Ruhlman and Robert K. Jansen
2. Plastid Gene Transcription: An Update on Promoters and RNA Polymerases Jennifer Ortelt and Gerhard Link 
3. Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene ExpressionRalph Bock
4. Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA SequencesElisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila, and Anil Day
5. Fluorescent Labeling and Confocal Microcopy of Plastids and StromulesMaureen R. Hanson, Patricia L. Conklin, and Amirali Sattarzadeh 
Part II Nicotiana Tabacum: The Model Species of Chloroplast Biotechnology 
6. Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome EngineeringPal Maliga, Tarinee Tungsuchat Huang, and Kerry Ann Lutz 
7. Nicotiana tabacum: An Update on PEG-Mediated Plastid TransformationAreli Herrera Díaz and Hans-Ulrich Koop 
8. Transformation of the Plastid Genome in Tobacco Suspension Cell CulturesJeffrey M. Staub 
9. Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinases Tarinee Tungsuchat-Huang and Pal Maliga 
10. Rubisco Engineering by Plastid Transformation and Protocols for Assessing ExpressionSpencer M. Whitney and Robert E. Sharwood
Part III Crop Specific Plastid Transformation Protocols
11. Plastid Transformation in Tomato: A Vegetable Crop and Model SpeciesStephanie Ruf and Ralph Bock
12. Stable Plastid Transformation of Petunia for Studies in Basic ScienceElena Martin Avila and Anil Day
13. Plastid Transformation in Potato: An Important Source of Nutrition and Industrial MaterialsVladimir T. Valkov, Daniela Gargano, Teodoro Cardi, and Nunzia Scotti 
14. Plastid Transformation of Poplar: A Model for Perennial TreesYuyong Wu, Ling Chang, Chunmei Jiang, Letian Xu, and Jiang Zhang 
15. Plastid Transformation in Lettuce (Lactuca sativa) by Biolistic DNA Delivery Tracey A. Ruhlman 
16. Plastid Transformation in Sugar Beet: An Important Industrial CropFrancesca De Marchis and Michele Bellucci
Part IV Plastid Transformation in Algae
17. A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtiiMarco Larrea-Alvarez, Rosanna Young, and Saul Purton
18. Regulated Chloroplast Gene Expression in ChlamydomonasJean-David Rochaix, Raymond Surzycki, and Silvia Ramundo 
Part V Plastid Transformation in Bryophyte
19. Plastid Transformation in Physcomitrella patens: An UpdateMamoru Sugita
20. Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L.Shota Chiyoda, Katsuyuki T. Yamato, and Takayuki Kohchi
21. Rapid and Modular DNA Assembly for Transformation of Marchantia ChloroplastsEftychios Frangedakis, Kasey Markel, Susana Sauret-Gueto, and Jim Haseloff 
Subject Index List…