Buch, Englisch, 196 Seiten, Format (B × H): 160 mm x 234 mm, Gewicht: 1050 g
Reihe: Methods in Molecular Biology
Buch, Englisch, 196 Seiten, Format (B × H): 160 mm x 234 mm, Gewicht: 1050 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-58829-645-0
Verlag: Humana Press
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.
Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
The History of Pyrosequencing®.- Pyrosequencing® Applications.- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®.- Pyrosequencing®.- Universal Primer Applications for Pyrosequencing®.- Allele Quantification and DNA Pooling Methods.- Multiplex Pyrosequencing® for DNA Variation Analysis.- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology.- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria.- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci.- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands.- Gene Copy Number Detection in Animal Studies.- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®.- Pyrosequencing® Genotype Storage Techniques.
