Microfluidic Techniques

Reviews and Protocols
2006. Auflage 2005
ISBN: 978-1-58829-517-0
Verlag: Humana Press

Buch, Englisch, Band 321, 248 Seiten, Format (B × H): 160 mm x 231 mm, Gewicht: 1200 g

Reihe: Methods in Molecular Biology

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Minteer, Shelley D

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Microfabrication Methods.- Overview of Advances in Microfluidics and Microfabrication.- to Microfabrication Techniques.- Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds.- Fabrication of Microelectrodes Using the Lift-Off Technique.- Laser Ablation as a Fabrication Technique for Microfluidic Devices.- Reversible, Room Temperature Bonding of Glass Devices for Microfluidics.- Biological Applications.- Solid-Phase Extraction in Packed Beds on Glass Microdevices.- Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems.- Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays.- Polymerase Chain Reaction in Miniaturized Systems:.- Polymerase Chain Reaction on Microchips.- A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes.- Microfluidic Ethanol Biobatteries on a Microchip.- Microfluidic Chambers for Cell Migration and Neuroscience Research.- Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells.- Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip.- Microscale Integrated Sperm Sorter.