Morgan / Yarmush Tissue Engineering Methods and Protocols

Erscheinungsjahr 2008
ISBN: 978-1-59259-602-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 18, 629 Seiten, eBook

Reihe: Methods in Molecular Medicine

In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

Zielgruppe

Research

Autoren/Hrsg.

Morgan, Jeffrey R.

Yarmush, Martin L.

Inhaltsverzeichnis

Materials.- Preparation of Collagen—Glycosaminoglycan Copolymers for Tissue Regeneration.- Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology.- Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering.- Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering.- Formation of Highly Porous Polymeric Foams with Controlled Release Capability.- Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents.- Preparation and Use of Thermosensitive Polymers.- In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing.- Fabrication and Implantation of Gel-Filled Nerve Guidance Channels.- Small Animal Surgical and Histological Procedures for Characterizing the Performance of Tissue-Engineered Bone Grafts.- Preparation and Use of Porous Poly(?-Hydroxyester) Scaffolds for Bone Tissue Engineering.- Cells.- Quantitative Assessment of Autocrine Cell Loops.- Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy.- Preparation of Immortalized Human Chondrocyte Cell Lines.- Clinical Applications.- Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells.- Culture and Identification of Autologous Human Articular Chondrocytes for Implantation.- Organogenesis of Skeletal Muscle in Tissue Culture.- Hepatocyte Primary Cultures.- Formation and Characterization of Hepatocyte Spheroids.- Isolation and Culture of Human Endothelial Cells.- Isolation and Culture of Microvascular Endothelial Cells.- Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures.- Methods to Isolate, Culture, and Study Osteoblasts.- Cryopreservation of Rat Hepatocytes in a Three-Dimensional CultureConfiguration Using a Controlled-Rate Freezing Device.- Cell Material Composites.- Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms.- Methods for Microencapsulation with HEMA-MMA.- Micropatterning Cells in Tissue Engineering.- Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen—GAG-Based Skin Substitutes.- Use of Skin Equivalent Technology in a Wound Healing Model.- Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis.- Development of a Bioartificial Liver Device.- Methods for the Implantation of Liver Cells.- Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture.- Design and Fabrication of a Small Caliber Hybrid Arterial Bioprosthesis.- Methods for the Immunoisolation and Transplantation of Pancreatic Cells.- Methods for the Study of Nerve Cell Migration and Patterning.- Measurement Techniques.- Estimating Number and Volume of Islets Transplanted Within a Planar Immunobarrier Diffusion Chamber.- Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions.- Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation.- Measuring Receptor-Mediated Cell Adhesion Under Flow.- In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium.- Quantitative Measurement of Shear-Stress Effects on Endothelial Cells.- Quantitative Modeling of Limitations Caused by Diffusion.