Morgan / Yarmush Tissue Engineering Methods and Protocols

1999
ISBN: 978-1-59259-602-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 18, 629 Seiten, eBook

Reihe: Methods in Molecular Medicine

In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

Zielgruppe

Research

Autoren/Hrsg.

Morgan, Jeffrey R.

Yarmush, Martin L.

Inhaltsverzeichnis

Part I. Materials. Preparation of Collagen-Glycosaminoglycan Copolymers for Tissue Regeneration, Lila Chamberlain and Ioannis V. Yannas. Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology, Susan J. Sofia, Philip R Kuhl, and Linda G. Griffith. Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering, Leoncio Garrido. Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering, Peter X. Ma and Robert Langer. Formation of Highly Porous Polymeric Foams with Controlled Release Capability: A Phase-Separation Technique, S. Kadiyala, H. Lo, and K. Leong. Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents, Timothy S. Girton, Nerendra Dubey, and Robert T. Tranquillo. Preparation and Use of Thermosensitive Polymers, Yi-Lin Cao, Clemente Ibarra, and Charles Vacanti. In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing, Amarpreet S. Sawhney and Jeffrey A. Hubbell. Fabrication and Implantation of Gel-Filled Nerve Guidance Channels, Ravi V. Bellamkonda and Robert F. Valentini. Small Animal Surgical and Histological Procedeures for Characterizing the Performance of Tissue-Engineered Bone Grafts, Kenneth S. James, Mark C. Zimmerman, and Joachim Kohn. Preparation and Use of Porous Poly(a-Hydroxyester) Scaffolds for Bone Tissue Engineering, Anna C. Jen, Susan J. Peter, and Antionios G. Mikos. Part II. Cells. Quantitative Assessment of Autocrine Cell Loops, Gregory Oehrtman, Laura Walker, Birgit Will, Lee Opresko, H. StevenWiley, and Douglas A. Lauffenburger. Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy, John A. Faulkner, Susan V. Brooks, and Robert G. Dennis. Preparation of Immortilized Human Chondrocyte Cell Lines, James R. Robbins and Mary B. Goldring. Part III. Clinical Applications. Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells, Sonya Shortkroff and Myron Spector. Culture and Identification of Autologous Human Articular Chondrocytes for Implantation, Ross Tubo and Francois Binette. Organogenesis of Skeletal Muscle in Tissue Culture, Herman Vandenburgh, Janet Shansky, Michael Del Tatto, and Joseph Chromiak. Hepatocyte Primary Cultures: Currently Used Systems and Their Applications for Studies of Hepatocyte Growth and Differentiation, George K. Michalopoulos. Formation and Characterization of Hepatocyte Spheroids, Julie R. Friend, Florence J. Wu, Linda K. Hansen, Rory P. Remmel, and Wei-Shou Hu. Isolation and Culture of Human Endothelial Cells, François Berthiaume. Isolation and Culture of Microvascular Endothelial Cells, Lisa Richard, Paula Velasco, and Michael Detmar. Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures, Paul C. Collins, Sanjay D. Patel, William M. Miller, and E. Terry Papoutsakis. Methods to Isolate, Culture, and Study Osteoblasts, Mechteld V. Hillsley. Cryopreservation of Rat Hepatocytes in a Three-Dimensional Culture Configuration Using a Controlled-Rate Freezing Device, Michael J. Russo and Mehmet Toner. Part IV. Cell Material Composites. Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms, Thomas M. S. Chang. Methods for Microencapsulation with HEMA-MMA, Shahab Lahooti and Michael V. Sefton. Micropatterning Cells in Tissue Engineering, Sangeeta N. Bhatia, Martin L. Yarmush, and Mehmet Toner. Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen-GAG-Based Skin Substitutes, Steven T. Boyce. Use of Skin Equivalent Technology in a Wound Healing Model, Michael A. Vaccariello, Ashkan Javaherian, Nancy Parenteau, and Jonathan A. Garlick. Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis, Daniel A. Medalie and Jeffrey R. Morgan. Development of a Bioartificial Liver Device, Linda K. Hansen, Julie R. Friend, Rory Remmel, Frank B. Cerra, and Wei-Shou Hu.