Visualization of Receptors In Situ

1. Auflage 2000
ISBN: 978-0-8493-0042-4
Verlag: CRC Press

Buch, Englisch, 204 Seiten, Format (B × H): 184 mm x 260 mm, Gewicht: 109 g

Reihe: Methods in Visualization

Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not encountered in the original research papers - helping the reader overcome the challenges associated with this powerful technique.

Zielgruppe

Professional

Autoren/Hrsg.

Moyse, Emmanuel

Krantic, Slavica M

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Physiologie

Inhaltsverzeichnis

LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.

Über Autor(innen)

Emmanuel Moyse, Slavica M Krantic