Nesbeth Synthetic Biology Handbook

Erscheinungsjahr 2016
ISBN: 978-1-4665-6848-8
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 336 Seiten

The Synthetic Biology Handbook explains the major goals of the field of synthetic biology and presents the technical details of the latest advances made in achieving those goals. Offering a comprehensive overview of the current areas of focus in synthetic biology, this handbook:

- Explores the standardisation of classic molecular bioscience approaches

- Addresses the societal context and potential impacts of synthetic biology

- Discusses the use of legacy systems as tools for new product development

- Examines the design and construction of de novo cells and genetic codes

- Describes computational methods for designing genes and gene networks

Thus, the Synthetic Biology Handbook provides an accurate sense of the scope of synthetic biology today. The handbook also affords readers with an opportunity to scrutinize the underlying science and decide for themselves what aspects of synthetic biology are most valuable to their research and practice.

Autoren/Hrsg.

Nesbeth, Darren N.

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie

Inhaltsverzeichnis

STANDARDISING BIOLOGY

Synthetic Biology: Culture and Bioethical Considerations
Introduction
Perceptions and Portrayals of Synthetic Biology in Popular Culture and Politics
Synthetic Biology in Academic Literature
Societal Risks and Responsibilities Posed by Synthetic Biology
Governance of Synthetic Biology
Conclusions
Acknowledgements
References

Synthetic Biology Standards and Methods of DNA Assembly
Introduction
Theory and Considerations in Double-Stranded DNA Assembly
Assembly Methods in Detail
Distributed Construct Assembly Methods
Building Combinatorial Complexity during Assembly
Emerging Technologies and Conclusions
Standardisation of DNA Assembly
References

Standardised Genetic Output Measurement
Introduction
Standardisation in Conventional Biology
Standardisation in Synthetic Biology
Adapting Current Techniques as Synthetic Biology Standards
Towards Synthetic Biology Standards for Protein Measurement
Conclusions
Acknowledgements
References

ENGINEERING BIOLOGY WITH LEGACY TOOLS

Bacterial Cells as Engineered Chassis
Introduction
Designing and Refactoring Bacterial Genes
Bacterial Synthetic Gene Networks
Genome-Scale Re-Engineering of Bacterial Cells
Engineering Periplasmic Space
Re-Imagining the Bacterial Surface and Glycocalyx
References

Eukaryotae Synthetica: Synthetic Biology in Yeast, Microalgae, and Mammalian Cells
Introduction
Remodelling Outer Cell Membranes as Engineered Chassis Surfaces
Programmable Organelles
Eukaryotic Genes and Their Synthetic Counterparts
Networks of Synthetic Genes in Mammalian Cells
Genome-Scale Engineering in Eukaryotes
Synthetic Biotherapeutics
References

Synthetic Plants
Tools and Resources
Food Security and Sustainable Agriculture
Plant Chassis Engineering to Improve Nutritive Value
Synthetic Biology and Green Biopharms
Fibre and Fuel
Future of Plant Synthetic Biology
References

CONSTRUCTING NEW BIOLOGIES

Theory and Construction of Semi-Synthetic Minimal Cells
Introduction
Autopoiesis and Minimal Life
Semi-Synthetic Approach to the Construction of Minimal Cells
Entrapment of Solutes inside Liposomes
Biotechnological Perspectives of Synthetic Cells
Concluding Remarks
Acknowledgements
References

Design Tools for Synthetic Biology
Introduction
Mathematical Modelling in Synthetic Biology
Part Registries
Device Languages
Modelling Languages and Tools
Network Optimisation
Part Engineering
DNA Editing Tools
CAD Tools and Workflow Systems
Summary
References

New Genetic Codes
Introduction
Available Orthogonal Pairs and Their Host Systems
Using UAAs to Encode Post-Translational Modifications
UAAs as Synthetic Biology Tools to Detect Interactions Occurring within Cells
Photo-Activatable UAAs
Bioorthogonal Chemistry
Orthogonal Ribosomes
Systemic Optimization of New Genetic Codes
References

Über Autor(innen)

Darren N. Nesbeth earned his BSc in molecular biology from University College London (UCL), UK, and his PhD in molecular cell biology from Imperial College, London, UK. He did postdoctoral work on scale up of gene therapy at King's College London, UK; fundamental cell biology at Imperial College; and industrially applied cell engineering at UCL. In the UCL Department of Biochemical Engineering, he applies synthetic biology to address industrial challenges in manufacturing of biopharmaceuticals and fine chemicals.