Odagaki / Borroto-Escuela Co-Immunoprecipitation Methods for Brain Tissue

Erscheinungsjahr 2018
ISBN: 978-1-4939-8985-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 144, 166 Seiten, eBook

Reihe: Neuromethods

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Odagaki, Yuji

Borroto-Escuela, Dasiel O.

Inhaltsverzeichnis

Co-Immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue.- Co-Immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor a at Postsynaptic Density in Brain Tissue.- Use of Co-Immunoprecipitation to Study Conformation-Specific protein Interactions of Oligomeric a-Synuclein Aggregates.- Using Co-Immunoprecipitation and Shotgun Mass Spectrometry for protein-Protein Interaction Identification in Cultured Human Oligodencrocytes.- Co-Immunoprecipition Analysis of GPCR Complexes in the Central Nerve System.- The Histoblot Technique: A Reliable Approach to Analyse Expression Profile of proteins and to Predict their Molecular Association.- Co-Immunoprecipitation Assay to Investigate the Interaction Strength between Synaptic Protein using COS-7 Cells.- Guanosine-5’-O-(3-[35S]thio)triphosphate ([35S]GTPS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-G Antibody in Mammalian Brain Membranes.- Co-Immunoprecipitation as a Useful Tool for Detection of G protein-Coupled Receptors Oligomers.- Isolation and Detection of G Protein–Coupled receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation using a Combined Brain Subcellular Fractionation/Co-Immunoprecipitation (co-IP) Procedures.- Co-Immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments.- Co-Immunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita during Aestivation.- Co-Immunoprecipitation (co-IP) of G Protein-Coupled Receptors (GPCR)-Receptor Tyrosine Kinases (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain.