Paulus / Altria / Klockow-Beck Analysis of Carbohydrates by Capillary Electrophoresis

1999
ISBN: 978-3-322-85020-1
Verlag: Vieweg & Teubner
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 3, 176 Seiten, eBook

Reihe: CHROMATOGRAPHIA CE-Series

Carbohydrates are ubiquitous. They come as monomers, oligomers and polymers with a startling variety of chemical, physical and material properties. They affect almost every as pect of human live. For example they are an essential part of our nutrition. Cellulose, a car bohydrate polymer, acts as a central material in civilization: as building material for housing and as paper for written communication. Scientifically, the interest in carbohydrates ranges from structural elucidation of simple carbohydrates to fundamental biochemical processes such as photosynthesis and glycolysis. Always, a deeper understanding of a particular aspect of carbohydrate chemistry was accompanied by new methods of measuring and characteri zing this class of molecules. During the last decades a better understanding of the roles of carbohydrates in biochemi cal pathways developed. It turned out, that carbohydrates attached to proteins in glycoprote ins are involved in a number of molecular recognition and targeting processes. This lead to a new research field: Glycobiology. However, unlike for proteins or nucleic acids, there is at this time no consistent theory how chemical composition and structure controls function and inherent information. In order to develop the database, on which new theories and scientific hypothesis are founded, analytical methods to separate compounds with an enormous struc tural diversity and only minor differences in chemical or physical properties has to be deve loped, preferably using only minute quantities.

Zielgruppe

Research

Autoren/Hrsg.

Paulus, Aran

Klockow-Beck, Antje

Altria, Kevin D.

Inhaltsverzeichnis

1 Introduction.- References.- 2 Capillary electrophoresis, instrumentation and modes.- 2.1 Electrophoretic mobility.- 2.2 Fused silica surface and electroosmotic flow.- 2.3 Plate number, migration time and resolution.- 2.4 Instrumentation.- 2.5 CE modes.- 3 Structures and properties of carbohydrates.- 3.1 Monosaccharides.- 3.2 Oligosaccharides.- 3.3 Polysaccharides.- 3.4 Glycoproteins.- 3.5 Glycosaminoglycans and Proteoglycans.- 3.6 Glycolipids.- 4 Separation and detection of carbohydrates in capillary electrophoresis.- 4.1 Separation and detection of non-derivatized carbohydrates.- 4.2 Separation and detection of derivatized carbohydrates.- 5 Applications.- 5.1 Mono- and disaccharides.- 5.2 Oligosaccharides.- 5.3 High molecular weight polysaccharides.- 5.4 Glycopeptides and Glycoproteins.- 5.5 Glycosaminoglycans.- 5.6 Glycolipids.- 5.7 Other glycoconjugates.

Über Autor(innen)

Dr. Aran Paulus arbeitet und forscht im Bereich Analytik/Chromatographie für die Firma Soane in Kalifornien, USA. Dr. Antje Klockow-Beck ist in gleicher Funktion bei der Firma R.W. Johnson in Schaffhausen, Schweiz, tätig.