Posudin / Massjuk / Lilitskaya Photomovement of Dunaliella Teod

2010
ISBN: 978-3-8348-9765-7
Verlag: Vieweg & Teubner
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Deutsch, 225 Seiten, eBook

The authors review the historical development and current state of the art in the biology of photomovement in algae and discuss problems in terminology and a logical basis for classification of photomovement in microorganisms. Their research has focused on two species of Dunaliella Teod., D. salina Teod. and D. viridis Teod., as the principal organisms investigated. The results of experimental investigations on the critical factors controlling and modulating photomovement are described and include the effects of various abiotic factors and critical aspects of photomovement.

Dr. Yuriy I. Posudin is a Professor at the National University of Life and Environmental Sciences of Ukraine.
Dr. Nadiya P. Massjuk was a Professor at the Institute of Botany, Academy of Sciences of Ukraine.
Dr. Galyna G. Lilitskaya is the Leading Engineer of the Institute of Botany, Academy of Sciences of Ukraine.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Posudin, Yuriy I.

Massjuk, Nadiya P.

Lilitskaya, Galyna G.

Inhaltsverzeichnis

1;Preface;7
2;Contents;9
3;List of Figures and Photographes;13
4;List of Tables;19
5;Introduction;21
6;Chapter 1 Photomovement of Algae – Historical Overview of Research and Current State of the Art
;24
7;Chapter 2 Terminology and the Fundamentals of Classification of Light-Induced Behaviour in Freely Motile Microorganisms
;31
7.1;2.1. Stare of the Art
;31
7.2;2.2. Paramelrical Classification Principles for Photomovement in Orga nisms
;36
7.3;2.3. Summary
;40
8;Chapter 3 Investigations with Species of Dunaliella Teod.
;41
8.1;3.1. History of the Discovery and Description of the Genus Dunuliella
;41
8.2;3.2. Characteristics of the Test Species
;45
8.3;3.3. Cultivation of the Species
;47
9;Chapter 4 Investigation of Photomovement in Dunaliella
;48
9.1;4.1. Methods of Investigating the Photomovement Parameters in Dnnaliella
;48
9.1.1;4.1.1. Experimental Installation
;48
9.1.2;4.1.2. Measuring the movement velocity of the cells
;49
9.1.3;4.1.3. Measuring the phototopotaxis
;49
9.1.4;4.1.4. Fourier Transform of Angular Distribution of the Cells
;51
9.2;4.2. Results of Measurement of Photomovement Parameters in Duna liella
;52
9.2.1;4.2.1. Photokinesis and Photokinetic Reactions
;52
9.2.2;4.2.2. Phototopotaxis
;55
9.2.3;4.2.3. Results of Fourier Transform of Angular Distribution of the Cells
;61
9.3;4.3. Summary;63
10;Chapter 5 Effect of Abiotic Factors on Photomovement Parameters of Dunaliella
;65
10.1;5.1. Effect of Temperature
;65
10.2;5.2. Effect of Electrical Fields
;66
10.3;5.3. Effect of pH
;68
10.4;5.4. Simultaneous Effect of Several Externat Factors
;71
10.5;5.5. Effect of Ultraviolet Radiation
;74
10.6;5.6. Effect of lonizing Radiation
;82
10.7;5.7. Summary
;85
11;Chapter 6 Structure of the Photoreceptor System
;86
11.1;6.1. Problems associated with Photoreception of AIgae
;86
11.2;6.2. Structure of Photoreceptor Systems in Green Algae
;86
11.3;6.3. Structure of the Photoreceptor System of Dunaliella
;91
11.3.1;6.3.1. Stigma
;91
11.3.2;6.3.2. Structure of the Photoreceptor
;92
11.3.3;6.3.3. Application of Two- Beam Irradiation to Dunaliellu Cells
;94
11.4;6.4. Summary
;96
12;Chapter 7 Identification of Photoreceptor Pigments
;97
12.1;7.1. Characteristics of Photoreceptor Pigments
;97
12.2;7.2. Identification or Photoreceptor Pigments in Euglena gracilis
;97
12.2.1;7.2.1. Euglena gracilis Photoreceptor Pigments
;97
12.2.2;7.2.2. Pigment Isolation
;98
12.2.3;7.2.3. Microspectrosphotometry and Microfluorometry of Pigments
;98
12.2.4;7.2.4. Determination of the Action Spectra for Photobiological Reactions
;100
12.2.5;7.2.5. Biochemical Methods
;101
12.2.6;7.2.6. Effect of Exogenous Chemicals on Photomovement
;102
12.2.7;7.2.7. Introduetion of Alternative Photoreceptor Pigments
;102
12.3;7.3. Identification of Photoreceptor Pigments in Green AIgae
;103
12.4;7.4. ldentification of the Photoreceptor Pigments in Dunaliella
;107
12.4.1;7.4.1. Analysis of the Phototopotaxis Action Spectra in Dunaliella
;107
12.4.2;7.4.2. Application of Lateral Ultraviolet lrradiation
;107
12.5;7.5. Summary
;110
13;Chapter 8 Mechanisms of Photoreception and Photoorientation in Dunaliella
;111
13.1;8.1. Photoreception and Photoorientation Mechanisms in Algae
;111
13.2;8.2. Diffractional Mechanisms of Photoreception and Photoorientation in Dunaliella
;114
13.3;8.3. Role of Proteins in Photoregulation Mechanisms in Flagellates
;117
13.4;8.4. Summary;118
14;Chapter 9 Sensory Transduction
;119
14.1;9.1. Methods for Investigation of Sensory Transduction
;119
14.2;9.2. Sensory Transduction in Euglena gracilis
;119
14.3;9.3. Sensory Transduction in Green AIgae
;121
14.4;9.4. Sensory Transduction in DunaIiella
;123
14.4.1;9.4.1. Methods of lnvestigation
;123
14.4.2;9.4.2. Effect of Calcium Ions
;123
14.4.3;9.4.3. Effect of lonophore A23 187
;125
14.4.4;9.4.4. Effect of Ouabain
;126
14.4.5;9.4.5. Effect of Cobalt Ions
;126
14.4.6;9.4.6. Effect of Cinnarizine and lsoptin
;127
14.4.7;9.4.7. Effect of Sodium Azide
;129
14.5;9.5. Summary;131
15;Chapter 10 Flagella Apparatus
;132
15.1;10.1. Structure
;132
15.2;10.2. Peculiarities of Flagellar Beating
;132
15.2.1;10.2.1. Flagella Beating in Euglena gracilis
;132
15.2.2;10.2.2. Flagella Beating in Green Algae
;133
15.2.3;10.2.3. Flagella Beating in Dunaliella
;134
15.3;10.3. Analysis of Flagellar Beating
;135
15.3.1;10.3.1. High-Speed Microcinematography
;135
15.3.2;10.3.2. Laser Light Scattering
;135
15.3.3;10.3.3. Method of Microphotometry
;136
15.4;10.4. Summary;137
16;Chapter 11 Applied Aspects of Aquatic Biomonitoring Vsing the Photomovement of Dunaliella
;138
16.1;11.1. Algae of Genus Dunaliella as Test-Objects
;138
16.2;11.2. Photomovement Parameters of Dunaliella as Test-Functions
;142
16.3;11.3. Effect of Surface-Active Substances on Photomovement of Dunaliella
;143
16.3.1;11.3.1. Characteristics of Surface-Active Substauces
;143
16.3.2;11.3.2. Effect of Various Types of Surface-Active Substances, their Combinations and Duration of Action on the Velocity of Movement in Dunaliella
;144
16.4;11.4. Investigation of the Effect of Heavy Metals on Photomovement in Dunaliella Using Laser Doppler Spectroscopy
;147
16.5;11.5. Vector Method of Biomonitoring
;150
16.5.1;11.5.1. Dependence of Vector R on the Type and Concentration of Surface-Active Substances
;152
16.5.2;11.5.2. Dependence of Vector R on the type and concentration of heavy metals
;154
16.5.3;11.5.3. Dependence of Vector R on the Type and Concentration of Pesticides
;161
16.5.4;11.5.4. Advantages of the Vector Method for Biomonitoring
;163
16.6;11.6. Summary;163
17;Chapter 12 Dunaliella Biotechnology
;164
17.1;12.1. Carotenoids, ß-carotene Biosynthesis and Stereoisomers
;164
17.2;12.2. Use of DunaIiella salina for the Commercial Production of ß-carotene
;166
17.3;12.3. Summary;171
18;Chapter 13 General Results and Perspectives of Further Investigations
;172
18.1;13.1. Problems of Terminology
;172
18.2;13.2. Phenomenology of Photomovement
;173
18.3;13.3. Photoreactions
;173
18.4;13.4. Photokinesis
;173
18.5;13.5. Phototopotaxis
;174
18.6;13.6. Motility
;175
18.7;13.7. Photoreceptor System
;176
18.8;13.8. Mechanisms of Photoreception
;176
18.9;13.9. Sensory Transduction of the Light Signal
;177
18.10;13.10. Importance of Data on Algal Photomovement for Related Fields of Science
;177
18.11;13.1.1. Applied lmportance of Data on the Photomovement of Algae
;180
19;References;181
20;Index of Latin Names;226
21;Subject Index;228
22;Author's Index;232
23;Chemicals – CAS nomenclature
;233
24;About the Authors
;235

Photomovement of Algae – Historical Overview of Research and Current State of the Art.- Terminology and the Fundamentals of Classification of Light-induced Behaviour in Freely Motile Microorganisms.- Investigations With Species of Dunaliella Teod..- Investigation of Photomovement in Dunaliella.- Effect of Abiotic Factors on Photomovement Parameters of Dunaliella.- Structure of the Photoreceptor System.- Identification of Photoreceptor Pigments.- Mechanisms of Photoreception and Photoorientation in Dunaliella.- Sensory Transduction.- Flagella Apparatus.- Applied Aspects of Aquatic Biomonitoring Using the Photomovement of Dunaliella.- Dunaliella Biotechnology.- General Results and Perspectives of Further Investigations.

Über Autor(innen)

Dr. Yuriy I. Posudin is a Professor at the National University of Life and Environmental Sciences of Ukraine. Dr. Nadiya P. Massjuk was a Professor at the Institute of Botany, Academy of Sciences of Ukraine. Dr. Galyna G. Lilitskaya is the Leading Engineer of the Institute of Botany, Academy of Sciences of Ukraine.