Raffaello / Vecellio Reane Calcium Signalling

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9018-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1925, 246 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Raffaello, Anna

Vecellio Reane, Denis

Inhaltsverzeichnis

High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels.- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling.- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators.- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes.- MCU Regulation in Lipid Bilayer and Electrophysiological Recording.- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer.- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter.- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana.- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes.- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes.- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle.- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis.- Ca2+-Dependent Regulation of TFEB and Lysosomal Function.- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum.- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2.- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes.- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes.- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer.- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells.- Purification of Functional F-ATP Synthase from Blue Native PAGE.