Schmitt-Kopplin Capillary Electrophoresis

Methods and Protocols
Erscheinungsjahr 2008
ISBN: 978-1-59745-376-9
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 384, 809 Seiten, eBook

Reihe: Methods in Molecular Biology

Capillary Electrophoresis: Methods and Protocols  presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them. In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques.  Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.

Zielgruppe

Research

Autoren/Hrsg.

Schmitt-Kopplin, Philippe

Inhaltsverzeichnis

Analyte-Oriented.- Determination of Small Ions With Capillary Electrophoresis and Contactless Conductivity Detection.- Metal Analysis With Capillary Zone Electrophoresis.- Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillary Electrophoresis.- Determination of Aliphatic Low-Molecular-Weight and Biogenic Amines by Capillary Zone Electrophoresis.- Capillary Electrophoretic Analysis of Organic Pollutants.- Capillary Electrophoresis With Laser-Induced Fluorescence.- Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Electrophoresis/Mass Spectrometry.- Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Capillary Electrophoresis.- Capillary Electrophoresis of Tropane Alkaloids and Glycoalkaloids Occurring in Solanaceae Plants.- Capillary Electrophoresis for Pharmaceutical Analysis.- Capillary Electrophoresis of Neutral Carbohydrates.- Capillary Electrophoresis of Sugar Acids.- Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.- Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry.- Separation of Dna by Capillary Electrophoresis.- Capillary Electrophoresis of Oxidative Dna Damage.- Capillary Electrophoresis of Gene Mutation.- Biomedical Applications of Amino Acid Detection by Capillary Electrophoresis.- Separation of Peptides by Capillary Electrophoresis.- Analysis of Proteins by Capillary Electrophoresis.- Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques.- Capillary Electrophoresis Separation of Microorganisms.- Methods-Oriented.- A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free-Zone Electrophoresis.- The CE Way of Thinking.- Adsorbed Cationic Polymer Coatings for Enhanced Capillary Electrophoresis/Mass Spectrometry of Proteins.- On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis.- On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis.- Free-Flow Electrophoresis System for Proteomics Applications.- Microemulsion Electrokinetic Chromatography.- Micellar Electrokinetic Chromatography of Aminoglycosides.- Capillary Electrochromatography and On-Line Concentration.- Analysis of Alkaloids in Single Plant Cells by Capillary Electrophoresis.- Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis.