Smolke The Metabolic Pathway Engineering Handbook

Tools and Applications
Erscheinungsjahr 2009
ISBN: 978-1-4200-7766-7
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 582 Seiten

This second volume of the Metabolic Pathway Engineering Handbook delves into evolutionary tools and gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels.

Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.

Zielgruppe

Biomedical engineers, researchers and advanced student across all the biological sciences including genetics, toxicology, pharmacology, natural product development.

Autoren/Hrsg.

Smolke, Christina

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie

Inhaltsverzeichnis

Volume II

Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert

Evolutionary
engineering of industrially important microbial phenotypes

Expanding
enzyme function and activity through molecular evolution

Evolving
the function of DNA and RNA regulatory regions through molecular evolution

Evolving
pathways and genomes for the production of natural and novel compounds

Models
predicting optimized evolutionary strategies

Section VII. Gene Expression Tools for Metabolic Pathway Engineering, Christina Smolke

Low-copy
number plasmids as artificial chromosomes

Chromosomal
engineering strategies

Regulating
gene expression through engineered RNA technologies

Tools
designed to regulate translational efficiency

Regulating
gene expression through post-translational modifications

Engineering
bifunctional enzymes for optimized metabolite transfer between sequential
conversion steps

Practical
pathway engineering – demonstration in integrating tools

Section VIII. Applications of Emerging Technologies to Metabolic
Engineering, Jay Keasling

Genome-wide
technologies: DNA and phenotypic microarrays, proteomics

Monitoring
and measuring the metabolome

Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee

Genome-scale
models, systems biology, and metabolic engineering

Cell-free
systems for metabolic engineering

Dynamic in
silico cell models

Appendix I: Tools for Experimentally Determining Flux through Pathways, Ralf Takors

Measuring
flux by isotope labeling

Tools for
measuring intermediate and product formation

Quantitative analysis of labeling data

Appendix II: Applications, Brian Pfleger and Brian Brazeau

Cofactor regulation in
biocatalytic redox reactions

Metabolic engineering for fine
chemical production

Metabolic engineering for drug
production

Metabolic engineering for
alternative fuels