Smolke The Metabolic Pathway Engineering Handbook

Fundamentals
1. Auflage 2009
ISBN: 978-1-4398-0297-7
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 678 Seiten

This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus Subtilis, Streptomyces, filamentous fungi, and mammalian cells using cell culture. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. For information on the complete handbook visit Cat No. 3923

Zielgruppe

Biomedical engineers, researchers and advanced student across all the biological sciences including genetics, toxicology, pharmacology, natural product development.

Autoren/Hrsg.

Smolke, Christina

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie

Inhaltsverzeichnis

Volume I

Introduction
and Preface to Handbook, Christina D. Smolke

Progression
of biological synthesis methods towards commercial relevance

The field
of metabolic engineering

An overview
of the metabolic pathway engineering handbook

Metabolic
engineering: looking towards the future

Section I. Cellular Metabolism, Vincent Martin

Transport
processes inside the cell

Energy generating
reactions

Biosynthesis
of important building blocks

Polymerization
of building blocks to macromolecules

Rare
metabolic conversions – harvesting diversity through nature

Section II. Balances and Reaction Models, Walter van Gulik

Growth
nutrients and diversity

Mass
balances, rates, and experiments

Data
reconciliation and error detection

Black box
models for growth and product formation

Metabolic
models for growth and product formation

A
thermodynamic description of microbial growth

Section III. Regulation of Metabolic Pathways, James Liao

Regulation
of specific pathways

Regulating
of secondary metabolism

Building networks as assemblies
of simpler control schemes

Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas

Metabolic
flux analysis

Metabolic
control analysis

Structure
and flux analysis of metabolic networks

Constraint-based
genome-scale models of cellular metabolism

Multi-scale
modeling of metabolic regulation

Validation
of metabolic models

Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen

Escherichia coli as a well-developed host for
metabolic engineering

Metabolic
engineering in yeast

Metabolic
engineering in Bacillus subtilis

Metabolic
engineering in Streptomyces

Metabolic
engineering in filamentous fungi

Metabolic
engineering in mammalian cells using cell culture