Confocal Microscopy

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-07-161401-3
Verlag: Springer US

Buch, Englisch, Band 2304, 363 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 910 g

Reihe: Methods in Molecular Biology

This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sohn, Haewon

Brzostowski, Joseph

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Biologische Mikroskopie
• Naturwissenschaften Chemie Analytische Chemie Magnetresonanz
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie

Inhaltsverzeichnis

Advances in Confocal Microscopy and Selected Applications.- Choosing Fluorescent Probes and Labeling Systems.- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy.- Microfabricated devices for confocal microscopy on biological samples.- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging.- High-resolution multicolor imaging of mitochondria in lymphocytes.- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy.- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy.- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum.- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis.- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy.- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy.- Method for Acute Intravital Imaging of the Large Intestine in Live Mice.- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism.- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry.- FLIM Imaging for Metabolic Studies in Live Cells.- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).