Nanoparticles in Biology and Medicine

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2012
ISBN: 978-1-4939-6247-1
Verlag: Humana Press

Buch, Englisch, Band 906, 555 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 1069 g

Reihe: Methods in Molecular Biology

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery.  In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Soloviev, Mikhail

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Biotechnologie: Mikrotechnologie, Nanobiotechnologie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Naturwissenschaften Biowissenschaften Proteinforschung
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie

Inhaltsverzeichnis

Procedures for the Synthesis and Capping of Metal Nanoparticles.- Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications.- Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis.- Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays.- Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues.- RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics.- Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins.- Synthesis of Semiconductor Nanoparticles.- Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature.- Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods.- In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing.- Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis.- The Use of Quantum Dots for Immunochemistry Applications.- Quantum Dots for Labelling Live Cells.- Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots.- The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry.- Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging.- Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging.- Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry.- Synthesis, Application, and Tracking ofMagnetic Carbon-Coated Nanoparticles in Plants.- Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”).- Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery.- Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery.- Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases.- Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency.- Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications.- Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection.- Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications.- Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins.- Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles.- Extraction of Organic Nanoparticles from Plants.- Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay.- Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression.- Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells.- Assessment of Toxicity of Nanoparticles Using Insects as Biological Models.- Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues.- Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles.- Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction.- Microscopy Imaging Methods for theDetection of Silver and Titanium Nanoparticles Within Cells.- Detection of TiO2 Nanoparticles in Cells by Flow Cytometry.- NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions.- Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis.- Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules.