Taira / Kataoka / Niidome Non-viral Gene Therapy

Gene Design and Delivery
1. Auflage 2006
ISBN: 978-4-431-27879-5
Verlag: Springer Tokyo
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 487 Seiten, eBook

Currently not available, will follow before Dec 30.

Zielgruppe

Research

Autoren/Hrsg.

Kataoka, Kazunori

Niidome, Takuro

Taira, Kazunari

Inhaltsverzeichnis

Molecules for Gene Therapy.- Recent Progress in Non-viral Gene Delivery.- Physical Chemistry of DNA-Carrier Complexes.- Liposomes.- Polymeric Gene Carriers.- Development of a Supramolecular Nanocarrier for Gene Delivery Based on Cationic Block Copolymers.- Chitosan.- Dendrimers as DNA Carriers.- Use of Synthetic Peptides for Non-viral Gene Delivery.- An Oligonucleotide Carrier Based on ?-1,3-Glucans.- Biological and Chemical Hybrid Vectors.- Controlled Gene Delivery.- Pharmacokinetics of Gene Delivery in Cells.- Active DNA Release from Complexes.- Controlled Release of DNA Using Thermoresponsive Polymers.- Active Transport of Exogenous Genes into the Nucleus.- Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation.- Functional Nucleotide Sequences Capable of Promoting Non-viral Genetic Transfer.- Shielding of Cationic Charge of the DNA Complex to Avoid Nonspecific Interactions for In Vivo Gene Delivery.- In Vivo Gene Transfer by Ligand-Modified Gene Carriers.- Optimizing Polyplexes into Synthetic Viruses for Tumor-Targeted Gene Therapy.- Gene Transfer and Target Diseases.- Clinical Trials Using Non-viral Gene Delivery Systems.- Current Protocols of Gene Delivery.- Evaluation of Size and Zeta Potential of DNA/Carrier Complexes.- Observation of DNA/Carrier Complexes Under Fluorescence Microscopy.- Synthesis of Chemically Modified Chitosan and a Study of Its Gene Transfection Efficiency.- Newly Designed DNA Fragments for Gene Correction.- Evaluation of Gene Expression In Vivo After Intravenous and Intraportal Administration of Lipoplexes.- Evaluation by Southern Blot Hybridization of DNA Administered with a Gene Carrier to Organs.- Evaluation of the Immune Response After Administration of Plasmid DNA-Non-viral Vector Complexes.- Gateway RNAi.-Design of Genes Based on Current RNA Technology.- Design of Intracellularly Active Ribozymes and siRNAs.- RNAi-Based Inhibition Specific for Mutant Alleles in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discrimination of Mutant and Wild-Type Alleles by siRNA.- In Vivo RNA Interference: Another Tool in the Box?.- Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription.- Intracellular Delivery of Nucleic Acids: Differences Between Transfection and siFection Reflect Differences Between DNA and RNA, and Between Oligodeoxynucleotides and Oligonucleotides.- In Vivo Antitumor Activity of a New Cationic Liposome siRNA Complex.- Enhancing RNAi with Synthetic RNA Duplexes.