Laboratory Manual of Reproductive Endocrinology and Assisted Reproductive Technology

Reproductive Endocrinology: A Practical, Hands-On Guide for Students
1. Auflage 2026
ISBN: 978-1-032-52821-2
Verlag: Taylor & Francis Ltd

Buch, Englisch, 224 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 453 g

The manual Reproductive Biology, Endocrinology and Assisted Reproductive Technology serve as a comprehensive, step-by-step guide for undergraduate and postgraduate students of Zoology, Life Sciences, Biotechnology, and Medical Sciences. It integrates fundamental concepts with advanced experimental approaches, beginning with animal handling and histological examinations, and progressing to modern techniques such as hormonal assays, protein profiling, oocyte retrieval, embryo grading, and infertility investigations. Detailed coverage of assisted reproductive technologies including IVF, ICSI, cryopreservation, and embryo transfer is complemented by discussions on ethics, legal frameworks, and the role of artificial intelligence (AI) in reproductive health. To support effective learning, the manual incorporates illustrations, labelled images, surgical video guide, glossary and references, making it an invaluable resource for both classroom teaching and online learning. Scientifically rigorous and practically oriented, it equips learners with conceptual clarity, technical skills, and ethical insight into the evolving field of reproductive biology.

Key Features:

• Step-by-step coverage of both basic and advanced experimental procedures in reproductive biology and endocrinology.

• Detailed modules on assisted reproductive technologies (ART), including IVF, ICSI, embryo grading, and cryopreservation.

• Integration of contemporary issues such as ethics, legal aspects, and artificial intelligence in reproductive health.

• Rich visual aids, including labelled images, illustrations, histological sections, and a step-by step surgical video guide, to support effective learning.

• Glossary of key terms and references to relevant literature for further study.

Zielgruppe

Academic, Postgraduate, Professional Reference, Undergraduate Advanced, and Undergraduate Core

Autoren/Hrsg.

Taneja, Jyoti

Bhardwaj, Priya

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Reproduktionsmedizin
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Wirbeltiere (Vertebrata) Säugetiere (Mammalia)
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung

Inhaltsverzeichnis

Chapter 1: Set up and maintenance of Rodent Animal House facility; Chapter 2: Display of major endocrine glands and reproductive organ system in male and female rats; Chapter 3: Microscopic examinations of histological sections using illustrations/permanent slides/photomicrographs of human/rat; Chapter 4: Surgical techniques: Principles of surgery in endocrinology; Chapter 5: Dissect and display of the hypothalamic-hypophyseal organ system in rats; Chapter 6: To study the estrous cycle by observing the vaginal smear of adult female rats (Sprague-Dawley) under normal conditions; Chapter 7: Study of Exfoliative cytology of human cervix and vagina through micrographs; Chapter 8: Study of Sperm counting, Sperm Vitality and Sperm Motility in rats; Chapter 9: Study the Effect of Cryptorchidism on Sperm Count and Motility in Rats; Chapter 10: Study of Protein Profile of Rat Ovarian Extract by SDS-PAGE; Chapter 11: Separation of steroid hormones by Thin layer Chromatography and Paper chromatography; Chapter 12: To determine and quantify glycogen/ascorbic acid levels in rat uterine tissue; Chapter 13: To determine and quantify fructose in the dorsolateral prostate and coagulating glands of rat; Chapter 14: To Estimate Total Estrogens in Human Serum Samples Using Competitive ELISA technique; Chapter 15: To Understand the Methodology of a Pregnancy Test Kit; Chapter 16: Birth control methods: Study of modern contraceptive devices; Chapter 17: Retrieval of oocytes by induced superovulation in immature mice; Chapter 18: Study of causes of infertility in males and females and infertility investigation using secondary data, models and pictographs; Chapter 19: Setting up and Study of IVF Laboratory Culture Conditions; Chapter 20: Oocyte scoring and embryo quality grading in IVF for successful implantation; Chapter 21: Study of Assisted Reproductive Techniques; Chapter 22: Assisted Reproductive Technology: Ethical principles and laws in India; Chapter 23: Artificial Intelligence tools for Detection and Management of Sexually Transmitted Infections and Women's Reproductive Health: A Practical Approach for Undergraduate Students.

Über Autor(innen)

Jyoti Taneja is an Assistant Professor, with over 15 years of teaching experience in the Department of Zoology at Daulat Ram College, University of Delhi. Her research focuses on reproductive health, infectious disease epidemiology, and the application of machine learning in disease biology. She has a particular interest in polycystic ovary syndrome (PCOS), menstrual health, and reproductive tract infections, exploring how lifestyle factors influence women’s reproductive well-being.

In addition to her work in reproductive health, Dr. Taneja applies AI-driven methods to improve diagnostic accuracy and support vaccine development. Her expertise in infectious disease epidemiology has been pivotal in understanding the dynamics of COVID-19, including disease severity, breakthrough infections, post-COVID sequelae, and vaccine hesitancy. Her research has contributed significantly to evidence-based public health policies and interventions, and is widely published in leading scientific journals.

Priya Bhardwaj is a Scientist in the Department of Pharmacology at the All India Institute of Medical Sciences (AIIMS), New Delhi. She obtained her B.Sc. (Hons.) in Zoology, M.Sc. in Zoology, and Ph.D. from the University of Delhi, India. During her Master’s degree, she specialized in reproductive biology and subsequently received training in clinical research methods in public health.

Her research paper got published in reputed National and International journals and has over five years of experience in conducting reproductive biology practicals for postgraduate students. Her research interests include proteomics-based cancer diagnostics, cardiovascular pharmacology, COVID-19, and reproductive health. Dr. Bhardwaj has also been recognized as a resource person in several workshops, refresher courses, and faculty development programs.