Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

1. Auflage 1997
ISBN: 978-0-8493-9233-7
Verlag: CRC Press

Buch, Englisch, 352 Seiten, Format (B × H): 182 mm x 265 mm, Gewicht: 880 g

The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of:History and backgroundPrinciples and theoryEquipment and reagentsProtocolsTroubleshootingApplicationsImprovementsResultsComparisons with other methodsFuture prospects and developmentsThis is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.

Autoren/Hrsg.

Taylor, Graham R.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung

Inhaltsverzeichnis

Introduction, G.R. TaylorArbitrarily Primed PCR Fingerprints, F. Mathieu-Daude, D. Ralph, and M. McClellandMinisatellite Analysis, G. Rysiecki, W.P. Childs, and N. DuxburyMicrosatellite Analysis, S. PayneARMS Analysis of Point Mutations, S. LittleSolid Phase Minisequencing, A.C. SyvanenOligonucleotide Ligation Assay, E.S. Winn-DeenCombined Single-Strand Conformation Polymorphism and Heteroduplex Analysis, A.J. WallaceDenaturing Gradient Gel Electrophoresis (DGGE), M. Loosekoot and R. FoddeCoupled Transcription-Translation, T. Hamzehleoi and S. WestPeptide Nucleic Acids, H. Orum, T Koch, M. Egholm, H. O'Keefe, and J. CoullOrphan Peak Analysis, M. Hattori, H. Hagiwara, and Y. SakakiSequence-Specific Gene Signatures Produced by LSSP-PCR, A.J.G. Simpson and S.D.J. PenaBisulphite Genomic Sequencing of Methylated Cytosines, S.J. Clark and M. FrommerDirect Sequencing of PCR Products, E. WerleHeterozygote Determination Using Automated DNA Sequencing Technology, M.N. KronickA Robust Sequencing Protocol for PCR Products, M.D. Robinson and K.J. LeachUse of MutY and Thymine Glycosylase to Detect Point Mutations, I.C. HsuMutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes, V.H. Laitinen, G.R. Taylor, S. Hamilton and P. FlickEnzyme Mismatch Cleavage-Bacterial Resolvases, R.D. Mashal and J. SklarChemical Cleavage of Mismatch in Heteroduplexes, J. ProsserComparison of Mutation Detection by Chemical Cleavage of Mismatch (CCM) and Enzyme Mismatch Cleavage (EMC), R. Youil, S.J. Ramus, and R.G.H. CottonAnalysis of DNA Sequence Using Biosensors, P. Nilsson, B. Persson, M. Uhlen, and P.A. NygrenRepeat Expansion Detection, C. Zander, C. Erickson Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. SchallingComparative Genomic Hybridization, A Kallioniemi, R. Karhu, and O.-P. KallioniemiLarge-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE), I.N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. HumphriesDNA Sequence Database Searches, Comparisons, and Primer Design, G. WilliamsAccessing Genome Data: The Human Genome Database, Internet, and WWW, G. WilliamsList of SuppliersIndex

Über Autor(innen)

Taylor\, Graham R.