Buch, Englisch, Band 2557, 816 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 1540 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2557, 816 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 1540 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-2641-2
Verlag: Springer US
This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.
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Weitere Infos & Material
Live Cell Imaging of Yeast Golgi Dynamics.- Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole.- Immunofluorescence as a Method to Study Golgi Organization in Larval Salivary Glands of Drosophila melanogaster.- Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgi.- Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells.- Generation of GM130 Conditional Knockout Mouse.- Monitoring Effects of Membrane Traffic via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts.- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.- Studying the Organization of the Golgi by Super-Resolution Microscopy.- Super-Resolution Live Imaging of Cargo Traffic Through the Golgi Apparatus in Mammalian Cells.- Studying Golgi Structure and Function by Thin Section TEM.- Algorithm for Modern Electron Microscopic Examination of the Golgi Complex.- High-Pressure Freezing Followed by Freeze-Substitution, An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking.- Negative Staining Electron Microscopy for Morpho-Functional Characterization of Purified Golgi Membranes from Mammalian Cells.- An Electron Tomographic Analysis of Giantin Deficient Golgi Proposes a New Function of the Golgin Protein Family.- Modeling the Cryo-EM Structure of the Exocyst Complex.- Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Software.- A Primer on Deep Learning-Based Cellular Image Classification of Changes in the Spatial Distribution of the Golgi Apparatus After Experimental Manipulation.- Reconstituting Vesicle Budding from the TGN and Immunoisolation of Vesicles Enriched with a Specific Cargo Client.- Common Assays in Mammalian Golgi Studies.- In Vitro Methods to Investigate the Disassembly of the Golgi Ribbon during t
