Wijffels Immobilized Cells

Erscheinungsjahr 2012
ISBN: 978-3-642-56891-6
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 261 Seiten, eBook

Reihe: Springer Lab Manual

used. Generally in the development of a process, research activity will start with the physiology of the immobilized cells and be concluded by scale-up and application. Guidelines for the different phases in research are given below. Springer Lab Manual about immobilized cells The Springer Lab Manual about immobilized cells is structured in such a way that guidelines that resulted from the conference 'Immobilized Cells: Basics and Applications' were followed and translated into some practical methods. As for the guidelines for immobilized cells a structure was chosen in which general aspects of immobilization were covered, the kinetics were described and engineering aspects were covered. Besides laboratory meth ods some simple calculation procedures have been given in this lab man ual. The combination of the practical methods with the calculations pro vide a tool for carrying out research with immobilized cells. The lab manual concludes with some recent, promising and practical cases of immobilized cells as examples of applications with potential.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Wijffels, Rene H.

Inhaltsverzeichnis

1 Characterization of Immobilized Cells; Introduction.- Immobilization.- 2 Description of the Support Material.- 3 Description of the Immobilization Procedures.- 4 Measurement of Density, Particle Size and Shape of Support.- 5 Mechanical Stability of the Support.- 6 Diffusion Coefficients of Metabolites.- Kinetics.- 7 Quantity of Biomass Immobilized, Determination of Biomass Concentration.- 8 Kinetics of the Suspended Cells.- 9 Diffusion Limitation.- 10 Micro-Electrodes.- 11 Biomass Gradients.- 12 NMR and Immobilized Cells.- Engineering.- 13 Immobilization at Large Scale by Dispersion.- 14 Immobilization at Large Scale with the Resonance Nozzle Technique.- 15 External Mass Transfer.- 16 Liquid Fluidization of Gel-Bead Particles.- 17 Gradients in Liquid, Gas or Solid Fractions.- 18 Support Material Stability at the Process Conditions Used.- Cases.- 19 Immobilized Cells in Food Technology: Storage Stability and Sensitivity to Contamination.- 20 Immobilized Cells in Bioremediation.- 2 Plasmid Stability in Immobilized Cells.- 22 Immobilization for High-Throughput Screening.