Immune Mediators in Cancer

Methods and Protocols
1. Auflage 2020
ISBN: 978-1-0716-0246-1
Verlag: Springer US

Buch, Englisch, Band 2108, 373 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 925 g

Reihe: Methods in Molecular Biology

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Zhu, Yan

Vancurova, Ivana

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Onkologie, Krebsforschung

Inhaltsverzeichnis

Part I: Detection of Immune Mediators in Cancer Cells and Tissues



1. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique

Maria Faresjö



2. Analysis of Circulating HMGB1 in Human Serum

Weiqiang Chen, Guoqiang Bao, Lin Zhao, and Haichao Wang



3. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus

Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, and Cong-Qiu Chu



4. Application of Immunohistochemistry in Basic and Clinical Studies

Aihua Li and Dong-Hua Yang



5. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma

Takaaki Kamatani



6. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum

Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, and Petr Vodicka



7. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence

Herui Wang, Ryan L. Pangilinan, and Yan Zhu



Part II: Cytokine Bioassays



8. Analysis of IFNg-Induced Migration of Ovarian Cancer Cells

Bijaya Gaire, Mohammad M. Uddin, Yue Zou, and Ivana Vancurova



9. Interleukin-8-Induced Invasion Assay in Triple Negative Bre