Bastos Biocalorimetry

Foundations and Contemporary Approaches
Erscheinungsjahr 2016
ISBN: 978-1-4822-4666-7
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 416 Seiten

Connecting past, present, and future instrument development and use, Biocalorimetry: Foundations and Contemporary Approaches explores biocalorimetry’s history, fundamentals, methodologies, and applications. Some of the most prominent calorimeter developers and users share invaluable personal accounts of discovery, discussing innovative techniques as well as special and original applications. Wide in scope, the book also covers calorimetry use on membranes, nucleic acids, and proteins and addresses both thermodynamics and kinetics.

The book begins with a look at the historical development of calorimeters needed for biological research. It then describes advanced approaches that use high-quality commercial calorimeters to study biochemical and other biological processes. It also shows how novel experimental designs and data analysis procedures are applied to proteins, DNA, membranes, and living matter.

Autoren/Hrsg.

Bastos, Margarida

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Physik Physik Allgemein
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)

Inhaltsverzeichnis

Introduction: Historical and Methodological Context
Introduction
Margarida Bastos

From Classical Thermochemistry to Monitoring of Living Organisms
Ingemar Wadsö

A Journey in the Land of Biocalorimetry: A Quest for Knowledge
Lee D. Hansen

An Odyssey in Calorimetric Development: From Past to Future
Jaak Suurkuusk, Malin Suurkuusk, and Peter Vikegard

Enzyme-Catalyzed Reactions
Robert N. Goldberg

Membrane Characterization and Partition to Membranes
Temperature-Induced and Isothermal Phase Transitions of Pure and Mixed Lipid Bilayer Membranes Studied by DSC and ITC
Alfred Blume

The Thermotropic Properties of Ceramides in Aqueous or Lipidic Environments
Alicia Alonso and Felix M. Goñi

Peptide–Membrane Interactions Studied by Isothermal Titration Calorimetry
Johannes Klingler and Sandro Keller

DSC Studies on the Modulation of Membrane Lipid Polymorphism and Domain Organization by Antimicrobial Peptides
Karl Lohner

Kinetics of the Interaction of Amphiphiles with Lipid Bilayers Using Isothermal Titration Calorimetry
Patrícia A.T. Martins and Maria João Moreno

Nucleic Acids and Proteins: Stability and Their Interactions with Ligands
Calorimetry of DNA: Energetic Basis of the Double Helix
Peter L. Privalov

Allostery and Cooperative Interactions in Proteins Assessed by Isothermal Titration Calorimetry
Adrian Velazquez-Campoy

Protein Stability in Crowded Environments
Michael Senske, Simon Ebbinghaus, and Christian Herrmann

Isothermal Titration Calorimetry and Fluorescent Thermal and Pressure Shift Assays in Protein–Ligand Interactions
Vytautas Petrauskas, Lina Baranauskiené, Asta Zubriené, and Daumantas Matulis

Joining Thermodynamics and Kinetics by kinITC
Philippe Dumas

Calorimetric Approaches to Studying Complex Protein Structure–Function–Stability Relationships in Conformational Diseases: The Case of Cystathionine ß-Synthase Angel L. Pey, Tomas Majtan, and Jan P. Kraus

Calorimetry as a Tool in Applied Fields
An Account in Calorimetric Research: From Fundamentals to Pharmaceuticals
Patrick Connelly

Calorimetry for Monitoring Mixed Bacterial Populations and Biofilms
Simon Gaisford

Isothermal Microcalorimetry for the Investigation of Clinical Samples: Past and Present
Olivier Braissant, Gernot Bonkat, and Alexander Bachmann

Calorimetric Assays for Heterogeneous Enzyme Catalysis: Hydrolysis of Cellulose and Biomass
Peter Westh and Kim Borch

Index

Über Autor(innen)

Margarida Bastos is an associate professor in the Department of Chemistry and Biochemistry at the University of Porto, where she leads the biocalorimetry research group. She is a reviewer for many international journals and is involved with European community evaluation of research projects and international mobility grants. Having calorimetry as her main expertise, she has also been using other biophysical techniques, such as small angle x-ray diffraction (SAXD), neutron scattering, fluorescence spectroscopy, microscopy (SEM, TEM, and confocal), to study antimicrobial peptide/membrane interactions, polymer/surfactant mixtures, hydrophobic and hydrophilic compounds, and hydration of biopolymers. She earned a PhD in thermochemistry from Lund University.