Buch, Englisch, 314 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 816 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 314 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 816 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4099-9
Verlag: Humana
This updated volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to ensure successful results in the further study of this vital field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Direct or DNA extraction-free amplification and quantification of foodborne pathogens.- The use of multiplex real time PCR for the simultaneous detection of foodborne bacterial pathogens.- Viability detection of foodborne bacterial pathogens in food environment by PMA-qPCR and by microscopy observation.- Electrochemical Detection of Nucleic Acids using 3D Graphene Screen Printed Electrodes.- Application of MinION long read sequencer for semi-targeted detection of foodborne pathogens.- Integrating MALDI-TOF mass spectrometry with machine learning techniques for rapid antimicrobial resistances screening of foodborne bacterial pathogens.- Assessment of viability of by flow cytometry. - Method to study the survival abilities of foodborne bacterial pathogens under food processing conditions.- Characterization of bacterial membrane fatty acid profiles for biofilm cells.- Comparing gene expression between planktonic and biofilm cells of foodborne bacterial pathogens through RT-qPC,- Characterization of Food-Borne Pathogens in Biofilms: A 4D Structural Dynamics Approach Using HCS-CLSM.- Zebrafish larvae microinjection and automated fluorescence microscopy for studying infection and the host immune response.- Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of hypervirulent in A Comprehensive Guide to Quality Assessment and Data Submission for Genomic Surveillance of Enteric Pathogens.- Genomic pipeline for analysis of mutational events in bacteria.- Using GWAS and Machine Learning to identify and predict genetic variants associated with foodborne bacteria phenotypic traits.- Identifying putative biomarkers of foodborne pathogens using a metabolomic approach.- Illumina MiSeq 16S rRNA Gene Library Preparation for Poultry Processing Microbiome Analyses.- Bioinformatic pipeline for profiling foodborne bacterial ecology and resistome from short-read metagenomics.
