Cantor / Smith Genomics

The Science and Technology Behind the Human Genome Project
1. Auflage 2004
ISBN: 978-0-471-46186-9
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 624 Seiten, E-Book

Reihe: Baker Lecture Series

A unique exploration of the principles and methods underlying theHuman Genome Project and modern molecular genetics andbiotechnology-from two top researchers
In Genomics, Charles R. Cantor, former director of the HumanGenome Project, and Cassandra L. Smith give the first integraloverview of the strategies and technologies behind the Human GenomeProject and the field of molecular genetics and biotechnology.Written with a range of readers in mind-from chemists andbiologists to computer scientists and engineers-the book beginswith a review of the basic properties of DNA and the chromosomesthat package it in cells. The authors describe the three maintechniques used in DNA analysis-hybridization, polymerase chainreaction, and electrophoresis-and present a complete exploration ofDNA mapping in its many different forms. By explaining both thetheoretical principles and practical foundations of modernmolecular genetics to a wide audience, the book brings thescientific community closer to the ultimate goal of understandingthe biological function of DNA. Genomics features:
* Topical organization within chapters for easy reference
* A discussion of the developing methods of sequencing, such assequencing by hybridization (SBH) in which data is read throughwords instead of letters
* Detailed explanations and critical evaluations of the manydifferent types of DNA maps that can be generated-includingcytogenic and restriction maps as well as interspecies cellhybrids
* Informed predictions for the future of DNA sequencing

Autoren/Hrsg.

Cantor, Charles R.

Smith, Cassandra L.

Inhaltsverzeichnis

DNA Chemistry and Biology.
A Genome Overview at the Level of Chromosomes.
Analysis of DNA Sequences by Hybridization.
Polymerase Chain Reaction and Other Methods for In Vitro DNAAmplification.
Principles of DNA Electrophoresis.
Genetic Analysis.
Cytogenetics and Pseudogenetics.
Physical Mapping.
Enhanced Methods for Physical Mapping.
DNA Sequencing: Current Tactics.
Strategies for Large-Scale DNA Sequencing.
Future DNA Sequencing Without Length Fractionation.
Finding Genes and Mutations.
Sequence-Specific Manipulation of DNA.
Results and Implications of Large-Scale DNA Sequencing.
Appendix.
Index.

Über Autor(innen)

CHARLES R. CANTOR, formerly principal scientist of the DOE HumanGenome Project, is the Chief Scientific Officer of Sequenom, Inc.,in San Diego. Dr. Cantor is on leave from Boston University, wherehe is Professor of Biomedical Engineering and Director of theCenter for Advanced Biotechnology.
CASSANDRA L. SMITH is Deputy Director of the Center for AdvancedBiotechnology and Professor of Biomedical Engineering, Biology, andPharmacology at Boston University.