Edwards Liposomes in Analytical Methodologies

Erscheinungsjahr 2016
ISBN: 978-981-4669-27-6
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 480 Seiten

Liposomes have been widely explored in the drug delivery realm over the past decades. Many of the properties that made them well suited for drug delivery applications, such as the internal space to encapsulate a large payload of hydrophilic molecules and the inherent protection from exterior stresses, have also been exploited in unique analytical methodologies. Such liposome-based methods offer advantages over existing technologies and provide approaches not possible with other systems. For example, hydrophobic membrane receptors, which otherwise often lose activity in aqueous systems, can be incorporated into the lipid bilayer to afford novel biorecognition strategies; the internal volume can be used to entrap high concentrations of hydrophilic molecules for signal amplification; and the protected contents can be triggered to release as a function of pH or temperature, affording flexibility in microfluidic designs. These properties lend themselves to versatile assay platforms ranging from high-throughput ELISA formats to point-of-care diagnostics to electrophoretic separations.

Liposomes in Analytical Methodologies provides up-to-date consideration of actively researched techniques, written by expert investigators in their respective fields from a perspective not readily available in the primary literature. The introductory chapter serves as a primer for readers entering the area as well as a refresher for those with existing background to understand the fundamentals and broad application of liposomes for analytical purposes. With topics ranging from liposome arrays that exhibit environment-dependent color changes to exquisite fluorescent imaging, readers will gain an appreciation for the versatile utility that liposomes can add to the analytical toolbox.

Autoren/Hrsg.

Edwards, Katie A.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie
• Naturwissenschaften Chemie Analytische Chemie

Inhaltsverzeichnis

The analytical utility of liposomes: from the past to the present; K. Edwards

Applications of liposomes in lateral flow assays; H. W. Wen et al.

Application of liposomes in proteome microarrays for the study of lipid-protein interactions; C. S. Chen et al.

Functional polydiacetylene liposomes as self-signaling and signal amplifying bio-and chemical sensor and sensor array; J. Kim et al.

Taking advantage of liposomes in microfluidic systems; A. J. Baeumner et al.

Redox liposomes in the development of electrochemical sensors; J. A. Ho, et al.

Coupling liposome-encapsulated nucleic acids with the polymerase chain reaction: a generic assay platform for high-sensitivity analyte detection; J. Mason et al.

Magnetic liposomes in imaging and analysis; K. Edwards

Liposome-based methodologies to assess pharmacokinetic parameters of drugs; M. Langner et al. Liposomes in capillary electromigration techniques; S. Wiedmer

Fluorescent dye-encapsulating liposomes for cellular visualization; A. Csiszár et al.