Gaudreau / Guillemette Histones
2. Auflage 2025
ISBN: 978-1-0716-4486-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Methods and Protocols
E-Book, Englisch, 292 Seiten
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4486-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to be comprehensive guide forresearchers in the field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
In vitro assembly of nucleosomes for binding/remodeling assays.- Ruthenium-catalyzed one-pot peptide ligation.- A method for H2A.Z/H2B dimer exchange within nucleosomes in vitro.- Automated sample preparation for the unbiased analysis of histone post-translational modifications via mass spectrometry.- Detection of ? -H2A.X for Rapid Assessment of Genotoxic Agents-Induced Double-Stranded DNA breaks by Immunofluorescence.- High Resolution Cryo-EM Analyses of Nucleosomes.- Correlative Electron spectroscopic Imaging (ESI) and electron tomography of chromatin.- Psoralen crosslinking-Chromatin endogenous cleavage assay to examine histone DNA interactions of active and inactive rRNA genes.- Genome-wide nucleosome mapping by H3Q85C-directed chemical cleavage in Saccharomyces cerevisiae.- Efficient in-house coupling of sample and library preparation for ChIP-seq of histone modifications in complex plant tissues.- Chromatin immunoprecipitation assays of histone modifications in Daphnia magna.- Single-cell epigenomic profiling with high-throughput droplet scChIP-seq.- Determining the Substrate Specificity of Lysine Methyltransferases.- Identification of chromatin readers using a peptide pull-down and mass spectrometry integrated approach.- Efficient, Conditional Depletion of Nuclear Proteins in Saccharomyces cerevisiae.- RNA Immunoprecipitation (RIP) from Purified Nuclei in Cells.
