E-Book, Englisch, Band 130, 416 Seiten, Format (B × H): 191 mm x 235 mm
Reihe: Methods in Cell Biology
Guo Sorting and Recycling Endosomes
1. Auflage 2015
ISBN: 978-0-12-802871-1
Verlag: Academic Press
Format: EPUB
Kopierschutz: 6 - ePub Watermark
E-Book, Englisch, Band 130, 416 Seiten, Format (B × H): 191 mm x 235 mm
Reihe: Methods in Cell Biology
ISBN: 978-0-12-802871-1
Verlag: Academic Press
Format: EPUB
Kopierschutz: 6 - ePub Watermark
Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane.
In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic, and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes.
This book brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling. This volume covers the approaches necessary to study the key components that mediate the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included.
- Provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane.
- Covers an increasingly appreciated field in cell biology
- Includes both established and new technologies
- Brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling
Fachgebiete
Weitere Infos & Material
1. Dynamic Imaging of the Recycling Endosomal Network in Macrophages
Adam A. Wall, Nicholas D. Condon, Jeremy C. Yeo, Nicholas A. Hamilton and Jennifer L. Stow
2. Analyzing the Functions of Rab11-Effector Proteins During Cell Division
Rytis Prekeris
3. Expression and Localization of Exocytic and Recycling Rabs from Magnaportheoryzae in Mammalian Cells
Yaoyao Qi, M. Caleb Marlin, Zhimin Liang, Dongmei Zhang, Jie Zhou, Zonghua Wang, Guodong Lu and Guangpu Li
4. Methods to Analyze Novel Liaisons Between Endosomes and Centrosomes
Hui-Fang Hung, Heidi Hehnly and Stephen Doxsey
5. Biochemical Analysis of Rabin8, the Guanine Nucleotide Exchange Factor for Rab8
Bin Wu, Juanfei Wang, Yuting Zhao and Wei Guo
6. Simple In vitro Assay of Arf GAPs and Preparation of Arf Proteins as Substrates
Pei-Wen Chen, Xiaoying Jian, Ruibai Luo and Paul A. Randazzo
7. An ACAP1 Coat Complex Acting in Endocytic Recycling
Jian Li and Victor W. Hsu
8. Biochemical Methods for Studying Kinetic Regulation of Arf1 Activation by Sec7
Brian C. Richardson and J. Christopher Fromme
9. Rab and Arf G Proteins in Endosomal Trafficking
Dipannita Dutta and Julie G. Donaldson
10. Qualitative and Quantitative Analysis of Endocytic Recycling
James B. Reineke, Shuwei Xie, Naava Naslavsky and Steve Caplan
11. Methods for Analyzing the Role of Phospholipase A2 Enzymes in Endosome Membrane Tubule Formation
Danielle N. Kalkofen, Paul de Figueiredo and William J. Brown
12. In vivo Analysis of Recycling Endosomes in C. elegans
Anbing Shi and Barth D. Grant
13. Monitoring Receptor Trafficking Following Retromer and WASH Deregulation
Douglas G. Osborne, Christine A Phillips-Krawczak and Daniel D. Billadeau
14. Quantitative Analysis of Endosome Tubulation and Microdomain Organization Mediated by the WASH Complex
Emmanuel Derivery and Alexis Gautreau
15. Rab GTPases and Kinesin Motors in Endosomal Trafficking
Cédric Delevoye and Bruno Goud
16. Measuring Receptor Recycling in Polarized MDCK Cells
Luciana Gallo and Gerard Apodaca
17. Structural and Functional Analysis of Endosomal Compartments in Epithelial Cells
Andres E. Perez Bay, Ryan Schreiner and Enrique Rodriguez-Boulan
18. Analyzing the Role of AP-1B in Polarized Sorting from Recycling Endosomes in Epithelial Cells
Heike Fölsch
19. Recycling of the Insulin Responsive Glucose Transporter Glut4 Regulated by the Small GTPase RalA and the Exocyst Complex
Chao Nie and Xiao-Wei Chen
20. GPCR Sorting at the Multivesicular Endosomes
Michael Robert Dores and JoAnn Trejo
21. Visualizing and Quantitating Sequence-Dependent GPCR Recycling
Shanna L. Bowman, Amanda L. Soohoo and Manojkumar A. Puthenveedu
22. Methods to Study Endocytic Trafficking of the EGF Receptor
Itziar Pinilla-Macua and Alexander Sorkin
Contributors
Gerard Apodaca, Departments of Medicine and Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA
Daniel D. Billadeau, Department of Biochemistry and Molecular Biology, Division of Oncology Research, Mayo Clinic, Rochester, MN, USA
Shanna L. Bowman, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
William J. Brown, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
Steve Caplan, Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA
Pei-Wen Chen, Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
Xiao-Wei Chen
Institute of Molecular Medicine, Peking University, Beijing, China
PKU-THU Center for Life Sciences, Peking University, Beijing, China
Nicholas D. Condon, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
Paul de Figueiredo
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
Department of Microbial Pathogenesis and Immunology, Norman Borlaug Center, Texas A&M University, College Station, TX, USA
Cédric Delevoye
Institut Curie, PSL Research University, Paris, France
CNRS UMR 144, Structure and Membrane Compartments, Paris, France
Emmanuel Derivery, Department of Biochemistry, Sciences II, University of Geneva, Geneva, Switzerland
Julie G. Donaldson, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Michael Robert Dores, Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, USA
Stephen Doxsey, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Dipannita Dutta, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Heike Fölsch, Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
J. Christopher Fromme, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA
Luciana Gallo, Departments of Medicine and Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA
Alexis Gautreau, Laboratoire de Biochimie, Unité Mixte de Recherche 7654, Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau Cedex, France
Bruno Goud
Institut Curie, PSL Research University, Paris, France
CNRS UMR 144, Molecular Mechanisms of Intracellular Transport, Paris, France
Barth D. Grant, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ, USA
Wei Guo, Department of Biology, University of Pennsylvania, Philadelphia, PA, USA
Nicholas A. Hamilton
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
Research Computing Centre, The University of Queensland, Brisbane, QLD, Australia
Heidi Hehnly
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY, USA
Department of Pharmacology, University of Washington, Seattle, WA, USA
Victor W. Hsu
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA, USA
Department of Medicine, Harvard Medical School, Boston, MA, USA
Hui-Fang Hung, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Xiaoying Jian, Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
Danielle N. Kalkofen, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
Guangpu Li
Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou, China
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Jian Li
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA, USA
Department of Medicine, Harvard Medical School, Boston, MA, USA
Zhimin Liang, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Guodong Lu, Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou, China
Ruibai Luo, Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
M. Caleb Marlin, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Naava Naslavsky, Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA
Chao Nie, Institute of Molecular Medicine, Peking University, Beijing, China
Douglas G. Osborne, Department of Biochemistry and Molecular Biology, Division of Oncology Research, Mayo Clinic, Rochester, MN, USA
Andres E. Perez Bay, Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA
Christine A. Phillips-Krawczak, Department of Biochemistry and Molecular Biology, Division of Oncology Research, Mayo Clinic, Rochester, MN, USA
Itziar Pinilla-Macua, Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Rytis Prekeris, Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA
Manojkumar A. Puthenveedu, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
Yaoyao Qi
Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou, China
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Paul A. Randazzo, Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
James B. Reineke, Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA
Brian C. Richardson, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA
Enrique Rodriguez-Boulan, Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA
Ryan Schreiner, Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA
Anbing Shi
Department of Medical Genetics, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
Institute for Brain Research, Huazhong University of Science and Technology, Wuhan, Hubei, China
Amanda L. Soohoo, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
Alexander Sorkin, Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Jennifer L. Stow, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
JoAnn Trejo, Department of Pharmacology, School of Medicine, University of...