Hall Regulators and Effectors of Small GTPases, Part D: Rho Family

1. Auflage 2000
ISBN: 978-0-08-049680-1
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 325, 547 Seiten, Web PDF

Reihe: Methods in Enzymology

This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Autoren/Hrsg.

Hall, Alan

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 325;10
4;Preface;16
5;Volumes in Series;18
6;Section I: Purification, Posttranslational Modification, and in Vitro Regulation;37
6.1;Chapter 1. Purification and Biochemical Characterization of TC10;39
6.2;Chapter 2. Expression and Purification of Rho/RhoGDI Com- plexes;51
6.3;Chapter 3. Bacterial Expressed DH and DH/PH Domains;61
6.4;Chapter 4. Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases;74
6.5;Chapter 5. Activation of Rac1 by Human Tiam1;87
6.6;Chapter 6. Activation of Rho GEF Activity by Ga13;97
6.7;Chapter 7. Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein;107
6.8;Chapter 8. Purification and Biochemical Activity of Salmonella Exchange Factor SopE;118
6.9;Chapter 9. Stimulation of Rho GDI Release by ERM Proteins;127
6.10;Chapter 10. Expression and Activity of Human Prenylcysteine- Directed Carboxyl Methyltransferase;137
6.11;Chapter 11. Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies;150
6.12;Chapter 12. Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin;161
7;Section II: Purification and Activity of GTPase Targets;173
7.1;Chapter 13. Measurement of GTPase · Effector Affinities;175
7.2;Chapter 14. Purification and in Vitro Activity of Rho-Associated Kinase;185
7.3;Chapter 15. Purification and in Vitro Activities of p21-Activated Kinases;191
7.4;Chapter 16. Stimulation of Phospholipase C-ß2 by Rho GTPases;203
7.5;Chapter 17. Regulation of Phospholipase D1 Activity by Rho GTPases;213
7.6;Chapter 18. In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac;226
7.7;Chapter 19. Activity and Regulation of p35/Cdk5 Kinase Complex;236
7.8;Chapter 20. Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins;250
7.9;Chapter 21. In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts;273
8;Section III: Analysis of Rho GTPase Function;291
8.1;Chapter 22. Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts;293
8.2;Chapter 23. Determination of GTP Loading on Rho;300
8.3;Chapter 24. Use and Properties of ROCK-Specific Inhibitor Y-27632;309
8.4;Chapter 25. Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells;321
8.5;Chapter 26. Expression of Rho GTPases Using Retroviral Vectors;331
8.6;Chapter 27. Expression of Rho GTPases Using Adenovirus Vectors;339
8.7;Chapter 28. In Vivo Activity of Wild-Type and Mutant PAKs;351
8.8;Chapter 29. Single Cell Assays for Rac Activity;363
8.9;Chapter 30. Effect of Rho GTPases on Na–H Exchanger in Mammalian Cells;370
8.10;Chapter 31. Actin Filament Assembly in Permeabilized Platelets;385
8.11;Chapter 32. Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells;392
8.12;Chapter 33. Isolation and in Vitro Contraction of Stress Fibers;405
8.13;Chapter 34. Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors;417
8.14;Chapter 35. Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR;425
9;Section IV: Biological Assays of Rho GTPase Function;437
9.1;Chapter 36. Rho-like GTPases in Tumor Cell Invasion;439
9.2;Chapter 37. Mouse Embryo Fibroblasts. A Genetic Model System for Studying Rho- and Ras-Dependent Cell Cycle Progression;451
9.3;Chapter 38. Analyses of Transforming Activity of Rho Family Activators;461
9.4;Chapter 39. Rho GTPases and Cell Migration–Fibroblast Wound Healing;477
9.5;Chapter 40. Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis;485
9.6;Chapter 41. Rho GTPases and Macrophage Phagocytosis;498
9.7;Chapter 42. Rho GTPases and Axonal Growth Cone Collapse;509
9.8;Chapter 43. Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation;518
9.9;Chapter 44. Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP;532
10;Author Index;541
11;Subject Index;567