Hall Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

1. Auflage 2001
ISBN: 978-0-08-049684-9
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, Band Volume 329, 553 Seiten

Reihe: Methods in Enzymology

Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Autoren/Hrsg.

Hall, Alan

Inhaltsverzeichnis

1;Front Cover;1
2;Regulators and Effectors of Small GTPases;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 329;12
6;Preface;18
7;Volumes in Series;20
8;Section I: Rab GTPases;40
8.1;Chapter 1. Structural Basis for Rab Function: An Overview;42
8.2;Chapter 2. Expression of Wild-Type and Mutant Green Fluorescent Protein–Rab1 for Fluorescence Microscopy Analysis;45
8.3;Chapter 3. Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase;53
8.4;Chapter 4. Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases;70
8.5;Chapter 5. Antibody and Oligonucleotide Probes to Distin- guish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms;78
8.6;Chapter 6. Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family;89
8.7;Chapter 7. Purification and Properties of Rab3 GDP/GTP Exchange Protein;98
8.8;Chapter 8. Purification and Properties of Rab3 GTPase-Activating Protein;106
8.9;Chapter 9. Rabphilin-3: A Target Molecule for Rab3 Small G Proteins;114
8.10;Chapter 10. Doc2? as Modulator of Ca2+ -Dependent Exocytosis;122
8.11;Chapter 11. Purification and Properties of Sec4p GTPase-Activating Protein;130
8.12;Chapter 12. Purification and Characterization of Yeast Exocyst Complex;139
8.13;Chapter 13. Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling;150
8.14;Chapter 14. Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays;159
8.15;Chapter 15 Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5;171
8.16;Chapter 16. Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis;184
8.17;Chapter 17. Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A;196
8.18;Chapter 18. Expression and Functional Analyses of Rab8 and Rab11a in Exocytic Transport from trans-Golgi Network;204
8.19;Chapter 19. Expression and Properties of Rab7 in Endosome Function;214
8.20;Chapter 20. Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport;227
8.21;Chapter 21. Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase ? Subunit;236
8.22;Chapter 22. Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters;249
8.23;Chapter 23. Expression and Properties of Rab25 in Polarized Madin–Darby Canine Kidney Cells;264
8.24;Chapter 24. Purification of TRAPPfrom Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart;273
9; Section II: ADP-Ribosylation Factor (ARF) GTPases;282
9.1;Chapter 25. Structural and Functional Organization of ADP- Ribosylation Factor (ARF) Proteins;284
9.2;Chapter 26. Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathway;286
9.3;Chapter 27. Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements;295
9.4;Chapter 28. Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1);303
9.5;Chapter 29. Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6);311
9.6;Chapter 30. Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)–Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3,4,5-Trisphosphate;318
9.7;Chapter 31. Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast;329
9.8;Chapter 32. Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor;339
9.9;Chapter 33. Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GAP1;346
9.10;Chapter 34. Expression, Analysis, and Properties of Yeast ADP- Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3;356
9.11;Chapter 35. Purification and Properties of ARD1, an ADP- Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain;363
9.12;Chapter 36. Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins;374
9.13;Chapter 37. Assay and Purification of Phosphoinositide-Depen- dent ADP-Ribosylation Factor (ARF) GTPase- Activating Proteins;382
9.14;Chapter 38. Biological Properties and Measurement of Phos- pholipase D Activation by ADP-Ribosylation Factor (ARF);394
9.15;Chapter 39. Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF)/Coatomer Function in Golgi Traffic;411
9.16;Chapter 40. Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes;418
9.17;Chapter 41. Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes;427
9.18;Chapter 42. ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex;444
9.19;Chapter 43. Purification, Properties, and Analysis of yARL3;456
9.20;Chapter 44. Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1);463
10;Section III: Sar GTPases;468
10.1;Chapter 45. Purification and Properties of Rat Liver Sec23–Sec24 Complex;470
10.2;Chapter 46. Purification of Functional Sec13p–Sec31p Complex, A Subunit of COPII Coat;477
11;Section IV: Dynamin GTPases;484
11.1;Chapter 47. Expression, Purification, and Functional Assays for Self-Association of Dynamin-1;486
11.2;Chapter 48. Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin;496
11.3;Chapter 49. Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments;507
11.4;Chapter 50. Interactions of Dynamin and Amphiphysin with Liposomes;517
11.5;Chapter 51. Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins;525
12;Section V: Septin GTPases;536
12.1;Chapter 52. Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis;538
13;Author Index;550
14;Subject Index;576