Hochfeld Producing Biomolecular Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers

Erscheinungsjahr 2010
ISBN: 978-1-4200-2131-8
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 408 Seiten

Containing authoritative and in-depth coverage, Producing Biomolecular Materials Using Fermenters, Bioreactors, and Biomolecular Synthesizers examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins. The book discusses the substance, character, makeup, and quality of the basic materials used in the production and downstream processing of boimolecular materials: raw materials, reagents, intermediates, and consumables. Dr. Hochfield gets right to the point, explaining just what must be done and how to do it effectively, then providing the formula necessary for reaching the required value, allowing you to simply plug-in your data and make protein. However, if you actually do need the origin and derivation of any given formula, you can go right to the extensive reference section in the Appendix, find the formula you need in the exact form that you need it, without having to wade through numerous pages of extraneous material. This classic work presents unparalled, detailed, and cutting-edge information on bioprocessing systems. A working reference and formulary for producing recombinant, bioactive, or other exotic proteins, peptides, and nucleic acids to specification, the text provides coverage of the related technologies, coupled with the extensive biotechnology glossary, manufacturer’s directories, extensive references, important formulae, charts, illustrations, comprehensive index, emphasis on practical techniques, time-proven methods, and essential applications. These features combine with its ingenious, easy-to-use layout to make it the resource you will consult on a regular basis.

Zielgruppe

Chemical engineers, production personnel in pharmacies, and biotech pharmacists

Autoren/Hrsg.

Hochfeld, William L.

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Pharmazeutische Technologie
• Wirtschaftswissenschaften Wirtschaftssektoren & Branchen Fertigungsindustrie Pharmaindustrie

Inhaltsverzeichnis

Introduction
Biotechnology
Bioproduction
The Bioprocess
Cell Lines
Cell-Culture Process
Separation, Recovery, Purification
Virus and Foreign-DNA Removal
Quality Assurance
Technology Overview
Biomolecular Foundations
Cellular Variation
Key Molecular Interactions
Base-Pair Complementarity
Genetic Coding
Interrupting Gene Segments
Sequence Determinations
Cloning
Synthesized DNA
Cell Lines
Expression Systems Introduction to the Bioprocess
Overview
Optimizing Product Yield
Kinetic Models
cGMP Standards
Contract Manufacturing
Complex Mixtures
Biomolecular Products
Joint Manufacturing
Downscaling to Scale Up
Large-Scale Bioprocesses
Scaling-Up In-House or in a Contract Facility
Lab-to-Pilot-Plant-to-Production
Increasing Bioprocess Scale
Differing Features at Larger Scale
Simulating Environment at Scale
Detailed Specifications
Impact on Downstream Bioprocessing
FDA Biologics Review Process
Regulating Biological Products Recombinant DNA Materials and Methods
Cloning
Applications
Screening and Selection
DNA Synthesis
Cell Lines
Expression Systems
Vector Construction
Prokaryotic and Eukaryotic Growth Characteristics
Expression Levels
Intra- vs. Extra-cellular Expression
Intracellular Expression
Extracellular Expression
Glycosylation
Mammalian Cell Glycosylation
Insect Cell Glycosylation
General Considerations
Further Post-translational Modifications
Economic Concerns
Regulatory Concerns
Manufacturers’ Directory Enzymes
Overview
Enzyme Protein Engineering
Transformation in Non-Aqueous Systems
Restriction Enzymes
Restriction-Enzyme Classes
Class I Restriction Enzymes
Class II Restriction Enzymes
Class III Restriction Enzymes
Restriction-Enzyme Specificity
Star Activity
Enzyme Families and Compatible Ends
Isoschizomers
Reverse Transcriptases
DNA and RNA Nucleases
Manufacturers’ Directory DNA-Amplification Reagents
Overview
Amplification by DNA Synthesis (PCR)
Amplification by RNA Transcription (TAS and 3SR
Amplification by Ligation (LAR, LCR, LAS)
Amplification by RNA Replication (Q Replicase)
Sequence-Based Amplification
Manufacturers Directory Cell-Culture Media
Overview
Prokaryotic Cell-Culture Medium
Eukaryotic Cell-Culture Medium
Defined Medium
Serum
Other Sera
Serum Contamination
Serum-Free (Defined) Medium
Cell-Culture Gels
Media Supplements
Attachment Factors
Buffers
Sodium
Earle’s Balanced Salt Solution
Growth Factors
Epidermal Growth Factor (EGF)
Fibroblast Growth Factors (FGF)
Acidic FGF
Basic FGF
Other Growth Factors
Lectins
Toxins
Transport Factors
Vitamins
Water
Manufacturers’ Directory Fermenters
Introduction to Fermentation
Historical Perspective
The Fermentation Process
Enzyme Fermentation in the Organic Phase
Cell Growth and Production
Factors Affecting Specific Growth Rate
Kinetic Models
Optimal Conditions
Recombinant Culture Kinetics
Fermenter Design
Kinetic Modeling of the Fermentation Process
STF Batch Scale Up
Power Number Method for STF Geometric Scale Up
Continuous-Culture Scale Up
Fermenter Manufacturers Directory Bioreactors
Introduction
Aseptic Conditions
Monoclonal Antibody Production
mAB Production in Ascites Fluid
Bioreactor Engineering and Design
Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program
Bioreactor Manufacturers Directory Biomolecular Synthesizers
DNA Synthesizers
Manufacturers Directory
Technology Overview
Peptides by Solid-Phase Synthesis
Manufacturers Directory
Biotechnology Glossary
References and Recommended Reading Index