Kannicht Posttranslational Modification of Proteins

Tools for Functional Proteomics
Erscheinungsjahr 2008
ISBN: 978-1-59259-181-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 194, 322 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Kannicht, Christoph

Inhaltsverzeichnis

Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry.- Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods.- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB).- Separation of N-Glycans by HPLC.- Enzymatic Sequence Analysis of N-Glycans.- Immunological Detection of O-GlcNAc.- Analysis of O-Glycosylation.- Characterization of Site-Specific Glycosylation.- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- Analysis of S-Acylation of Proteins.- Immunoblotting Methods for the Study of Protein Ubiquitination.- Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins.- Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry.- Analysis of Tyrosine-O-Sulfation.- ?-Amidated Peptides: Approaches for Analysis.- ?-Glutamate and p-Hydroxyaspartate in Proteins.- Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides.- Lysine Hydroxylation and Crosslinking of Collagen.- Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications.- 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.