Peeters Protides of the Biological Fluids

Proceedings of the Eighteenth Colloquium, Bruges, 1970
1. Auflage 2013
ISBN: 978-1-4831-4632-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 592 Seiten, Web PDF

Protides of the Biological Fluids contains the proceedings of the XVIIIth Colloquium held in Bruges, Belgium in 1970. The papers focus on protides of the biological fluids, with emphasis on three main topics related to protein chemistry: proteins during development and the recurrence of fetal proteins in cancer; protein-protein interactions; and trends and technical refinements in the insolubilization of proteins. This book is comprised of 95 chapters and begins with an assessment of the relationships and functions of the apoprotein and lipid moieties of lipoproteins, followed by a discussion on the characteristics and structure of the alpha-fetoproteins. The protein patterns in the post-natal period, as well as specific patterns encountered during development of the various organs, are then considered. Subsequent chapters explore the recurrence of various fetoproteins in hepatoma and in tumors of the digestive system; protein-protein interactions occurring during association or aggregation of some proteins such as myosin, fibrin, and casein; and preparation of insolubilized enzymes, together with the isolation and purification of antibodies. The final section is devoted to immunological studies and methods such as thin-layer ultracentrifugation. This monograph will be a useful resource for biologists and chemists alike.

Autoren/Hrsg.

Peeters, H.

Inhaltsverzeichnis

1;Front Cover;1
2;Protides of the Biological Fluids;4
3;Copyright Page;5
4;Table of Contents;6
5;Preface;16
6;Acknowledgements;17
7;ACADEMIC LECTURE;18
7.1;The Apoprotein and Lipid Moieties of the Lipoproteins;20
7.1.1;I. CHARACTERIZATION OF PLASMA LIPOPROTEINS;21
7.1.2;II. THE LIPID MOIETY OF LIPOPROTEINS;23
7.1.3;III. THE PROTEIN MOIETY OF LIPOPROTEINS;31
7.1.4;IV. LIPOPROTEIN MODELS;40
7.1.5;REFERENCES;42
8;SECTION A: Proteins During Development;44
8.1;Chapter 1. Variations on a Theme in Protein Structure;46
8.1.1;ACKNOWLEDGEMENTS;52
8.1.2;REFERENCES;52
8.2;Chapter 2. Some Physicochemical Characteristics of Human a1-Fetoprotein;54
8.2.1;MATERIAL AND METHODS;54
8.2.2;RESULTS;55
8.2.3;REFERENCES;59
8.3;Chapter 3. Purification and Chemical Characterization of Human Alpha-Fetoprotein;60
8.3.1;SUMMARY;64
8.3.2;REFERENCES;64
8.4;Chapter 4. Fetoprotein Level Development in the First Days of Life of Some Mammals;66
8.4.1;REFERENCES;71
8.5;Chapter 5. HL-A Antigens in Human Foetuses;72
8.5.1;INTRODUCTION;72
8.5.2;MATERIALS AND METHODS;72
8.5.3;RESULTS;73
8.5.4;DISCUSSION;76
8.5.5;ACKNOWLEDGEMENTS;78
8.5.6;REFERENCES;78
8.6;Chapter 6. Fetoproteins in Different Species of Mammals;80
8.6.1;MATERIALS AND METHODS;80
8.6.2;RESULTS;81
8.6.3;REFERENCES;89
8.7;Chapter 7. Development of the Plasma Protein Pattern in the Fetal Guinea Pig as shown by Gel Electrofocusing/Electrophoresis;92
8.7.1;INTRODUCTION;92
8.7.2;MATERIALS AND METHODS;92
8.7.3;RESULTS AND DISCUSSION;92
8.7.4;REFERENCES;95
8.8;Chapter 8. Appearance and Disappearance of Organ-specific Components during Kidney Tubulogenesis in Chick and Quail Embryos;96
8.8.1;MATERIAL AND METHODS;97
8.8.2;RESULTS;97
8.8.3;DISCUSSION AND CONCLUSIONS;101
8.8.4;SUMMARY;101
8.8.5;REFERENCES;102
8.9;Chapter 9. The Effect of Cellular Differentiation on the Mannosyl-transferase Activity of Chick Embryo Cells;104
8.9.1;REFERENCES;107
8.10;Chapter 10. Ontogenesis of Lysozyme in Man and Other Mammals;108
8.10.1;REFERENCES;110
8.11;Chapter 11. Ontogenetic Sequence of Serum Esterases in Some Mammals;112
8.11.1;MATERIALS;112
8.11.2;METHODS;112
8.11.3;RESULTS;113
8.11.4;DISCUSSION;114
8.11.5;ACKNOWLEDGEMENTS;118
8.11.6;REFERENCES;118
8.12;Chapter 12. Isoenzyme Studies in Human Embryonic Tissues and Cell Cultures;120
8.12.1;I. MATERIAL AND METHODS;120
8.12.2;II. RESULTS;120
8.12.3;ACKNOWLEDGEMENTS;124
8.12.4;REFERENCES;124
8.13;Chapter 13. Synthesis of Embryonic Hemoglobins in Mouse Embryos;126
8.13.1;REFERENCES;129
8.14;Chapter 14. Active Immunization of Tadpoles against Frog Hemoglobin and Developmental Consequences;130
8.14.1;SUMMARY;132
8.14.2;ACKNOWLEDGEMENTS;132
8.14.3;REFERENCES;133
8.15;Chapter 15. Changes in the Protein Composition of the Mammalian Lens during Embryonic Development and Ageing, with Special Reference to the Proteins of Low Molecular Weight;134
8.15.1;REFERENCES;137
8.15.2;Chapter 16. Protein Linked Carbohydrates in the Developing Corneal Stroma of the Cattle Embryo;138
8.15.3;DISCUSSION;140
8.15.4;REFERENCES;142
8.16;Chapter 17. resence of IgG Subclasses and Clq in Human Cord Sera;144
8.16.1;MATERIALS AND METHODS;144
8.16.2;RESULTS;145
8.16.3;DISCUSSION;147
8.16.4;REFERENCES;148
8.17;Chapter 18. Biochemical Particularities of Glucose-6-phosphate-dehydrogenase (G.6PD) in Umbilical Cord Blood;150
8.17.1;MATERIAL AND METHODS;150
8.17.2;RESULTS;151
8.17.3;DISCUSSION;154
8.18;Chapter 19. Monoclonal Immunoglobulins in Children;156
8.18.1;METHODS;156
8.18.2;PATIENTS;156
8.18.3;COMMENT;157
8.18.4;SUMMARY;159
8.18.5;REFERENCES;159
8.19;Chapter 20. A Pharmacokinetic Study of Intramuscular Injections of IgA and IgM Globulins in Newborn Children and Patients with an ADS {Antibody Deficiency Syndrome);162
8.19.1;REFERENCES;166
8.20;Chapter 21. Alpha1-Antitrypsin and Plasminogen Plasma Levels in Newborns;168
8.20.1;REFERENCES;169
8.21;Chapter 22. Postnatal Development of Tissue-specific Antigens of the Male Reproductive Accessory Glands;170
8.21.1;MATERIALS AND METHODS;170
8.21.2;RESULTS;171
8.21.3;DISCUSSION;174
8.21.4;REFERENCES;174
8.22;Chapter 23. Incorporation of the APR Sialoglycoproteins in Granulomatous Tissues;176
8.22.1;DISCUSSION;178
8.22.2;CONCLUSION;178
8.22.3;REFERENCES;178
8.23;Chapter 24. Immunological Studies of Detoxifying Enzymes in Postnatal Development of Rat Liver Microsomes;180
8.23.1;RESULTS;180
8.23.2;CONCLUSIONS;183
8.23.3;ACKNOWLEDGEMENT;183
8.23.4;SUMMARY;183
8.23.5;REFERENCES;184
8.24;Chapter 25. The lntravascular Mass of 21 Serum Proteins in Normal Mature and Premature Children;186
8.24.1;INTRODUCTION;186
8.24.2;METHODS;186
8.24.3;MATERIAL;186
8.24.4;RESULTS;187
8.24.5;DISCUSSION;190
8.24.6;ACKNOWLEDGEMENT;191
8.24.7;REFERENCES;191
8.25;Chapter 26. A Polynomial Expression for the Serum Concentrations of 21 Serum Proteins from 1 to 93 Years of Age in Normal Females and Males;192
8.25.1;INTRODUCTION;192
8.25.2;METHODS;192
8.25.3;MATERIAL;192
8.25.4;STATISTICAL METHODS;193
8.25.5;RESULTS;197
8.25.6;DISCUSSION;199
8.25.7;SUMMARY;200
8.25.8;ACKNOWLEDGEMENT;200
8.25.9;REFERENCES;200
8.26;Chapter 27. Rat Brain Proteins and Glycoproteins during Development;202
8.26.1;MATERIALS AND METHODS;202
8.26.2;RESULTS AND DISCUSSION;203
8.26.3;REFERENCES;205
8.27;Chapter 28. Early Malnutrition and Rat Brain Proteins;206
8.27.1;MATERIALS AND METHODS;206
8.27.2;RESULTS AND DISCUSSION;206
8.27.3;REFERENCES;208
8.28;Chapter 29. Catabolism of Proteins in Developing Rat Brain;210
8.28.1;MATERIAL AND METHODS;210
8.28.2;RESULTS AND DISCUSSION;210
8.28.3;REFERENCES;213
8.29;Chapter 30. Developmental Changes in Rat Brain;214
8.29.1;MATERIALS AND METHODS;214
8.29.2;RESULTS AND DISCUSSION;215
8.29.3;ACKNOWLEDGEMENTS;218
8.29.4;REFERENCES;218
9;SECTION B: Recurrence of Foetal Proteins in Cancer;220
9.1;Chapter 31. Introductory Remarks on Foetal Proteins and Their Reappearance in Tumors;222
9.2;Chapter 32. a-Fetoprotein Production by Normal Liver and Liver Tumors;224
9.2.1;aFP SYNTHESIS DURING THE LIVER REGENERATION;224
9.2.2;CELLULAR BASIS OF aFP PRODUCTION;228
9.2.3;REFERENCES;230
9.3;Chapter 33. Transitory Liver Antigens and Primary Hepatoma;232
9.3.1;REFERENCES;236
9.4;Chapter 34. Use of the a-Feto Protein Test for the Diagnosis of Primary Liver Cancer;238
9.4.1;MATERIAL AND METHODS;238
9.4.2;RESULTS AND DISCUSSION;238
9.4.3;ACKNOWLEDGEMENTS;241
9.4.4;REFERENCES;241
9.5;Chapter 35. Incidences of Three Different Fetal Proteins in Sera of Patients with Primary Hepatoma;242
9.5.1;MATERIALS AND METHODS;242
9.5.2;RESULTS;243
9.5.3;CONCLUSION;247
9.5.4;REFERENCES;247
9.6;Chapter 36. Resurgence of Two Fetal Type Aldolases in Hepatoma;248
9.6.1;MATERIAL AND METHODS;248
9.6.2;RESULTS;249
9.6.3;DISCUSSION;254
9.6.4;SUMMARY;254
9.6.5;ACKNOWLEDGEMENTS;255
9.6.6;REFERENCES;255
9.7;Chapter 37. Assessment of Foetal and Cord Blood, Amniotic Fluid and Hepatoma Patients' Blood for three Foetal Antigens;256
9.7.1;MATERIAL AND METHODS;256
9.7.2;RESULTS;256
9.7.3;DISCUSSION;259
9.7.4;SUMMARY;259
9.7.5;REFERENCES;259
9.8;Chapter 38. Occurrence of a1-Fetoprotein in Some Infants Suffering from Hepatopathy;260
9.8.1;REFERENCES;263
9.9;Chapter 39. Serum Alpha-Foetoprotein (af) in Patients with Hepatoma from Djakarta;264
9.9.1;MATERIAL AND METHODS;264
9.9.2;PATIENTS;265
9.9.3;RESULTS;265
9.9.4;DISCUSSION;266
9.9.5;REFERENCES;266
9.10;Chapter 40. Systematic a-Foetoprotein Research in 2500 Sera—Results;268
9.10.1;MATERIAL AND METHODS;269
9.10.2;RESULTS;269
9.10.3;CONCLUSION;270
9.10.4;ACKNOWLEDGEMENT;271
9.10.5;REFERENCES;271
9.11;Chapter 41. Alpha-foetoprotein, Alpha2-globulin and a Beta-globulin in Various Rat Tumours;272
9.11.1;MATERIAL AND METHODS;272
9.11.2;DISCUSSION;274
9.11.3;ACKNOWLEDGEMENTS;275
9.11.4;REFERENCES;275
9.12;Chapter 42. Immunochemical and Enzymatic Study of Human Digestive Tumours Maintained in Organ Culture;276
9.12.1;MATERIAL AND METHOD;276
9.12.2;RESULTS;277
9.12.3;DISCUSSION;282
9.12.4;CONCLUSION;282
9.12.5;BIBLIOGRAPHY;283
9.13;Chapter 43. Carcinoembryonic Antigen in Human Digestive Tumors;284
9.13.1;MATERIAL AND METHODS;284
9.13.2;RESULTS;285
9.13.3;DISCUSSION;292
9.13.4;BIBLIOGRAPHY;293
9.14;Chapter 44. Abdominal Lymphoma and a-Chain Disease: Report of Three Cases;294
9.14.1;MATERIAL AND METHODS;294
9.14.2;RESULTS;294
9.14.3;DISCUSSION;295
9.14.4;REFERENCES;297
9.15;Chapter 45. Demonstration of a Cancer Related Antigen in the Human Adult Colonic Mucosa;298
9.15.1;MATERIAL AND METHODS;298
9.15.2;RESULTS;299
9.15.3;DISCUSSION;299
9.15.4;REFERENCES;301
9.16;Chapter 46. The Occurrence of Certain Carcino-Embryonic Antigens in Man;302
9.16.1;MATERIALS;302
9.16.2;METHODS;302
9.16.3;RESULTS;303
9.16.4;SUMMARY;305
9.16.5;REFERENCES;305
9.17;Chapter 47. The Third Fraction of Complement in Healthy and Cancer Bearing Humans;306
9.17.1;SUMMARY;310
9.17.2;ACKNOWLEDGEMENTS;310
9.17.3;REFERENCES;310
9.18;Chapter 48. Basic Proteins of Lymphocytic Cells Derived from Human Leukemia and Other Lymphoproliferative Disorders;312
9.18.1;INTRODUCTION;312
9.18.2;EXPERIMENTAL;312
9.18.3;RESULTS AND DISCUSSION;313
9.18.4;REFERENCES;316
9.19;Chapter 49. A Comparative Study of Serum Immunoglobulins in the Principal Lymphoproliferative Disorders;318
9.19.1;I. METHODS;318
9.19.2;II. CHOICE OF SUBJECTS;318
9.19.3;III. RESULTS;320
9.19.4;IV. DISCUSSION;321
9.19.5;CONCLUSION;323
9.19.6;REFERENCES;323
10;SECTION C: Protein-Protein Interactions;324
10.1;Chapter 50.The Conformation of DNA;326
10.1.1;REFERENCES;332
10.2;Chapter 51. Dimerization of Skeletal Myosin in High Salt: Analysis of Reversible Association in a Highly Nonideal Protein System;334
10.2.1;RESULTS;335
10.2.2;DISCUSSION;342
10.2.3;REFERENCES;342
10.3;Chapter 52. Influence of Hydrostatic Pressure on the Reversible Polymerization of Fibrin Monomers;344
10.3.1;REFERENCES;347
10.4;Chapter 53. Galactothermin—Reversibly Heat-Precipitable Protein of Human Milk;348
10.4.1;EXPERIMENTAL;348
10.4.2;CONCLUSION;353
10.4.3;REFERENCES;353
10.5;Chapter 54. The Recombination of Heavy (H-) and Light (L-) Polypeptide Chains of Equine Antilymphocytic Globulin;356
10.5.1;INTRODUCTION;356
10.5.2;RESULTS;356
10.5.3;SUMMARY;358
10.5.4;REFERENCES;358
10.6;Chapter 55. The Association of as1-Casein at pH 2.5;360
10.6.1;INTRODUCTION;360
10.6.2;MATERIALS AND METHODS;360
10.6.3;RESULTS AND DISCUSSION;360
10.6.4;SUMMARY;363
10.6.5;REFERENCES;363
10.7;Chapter 56. The Phenomenon of Nonspecific Antibody Adhesion onto Antigen-Antibody Complex (Matuhasi-Ogata Phenomenon);364
10.7.1;DISSOCIATION OF ANTIBODIES NONSPECIFICALLY ADHERED ONTO THE RED CELL COLUMN COATED WITH SPECIFIC ANTIBODIES;364
10.7.2;NONSPECIFIC ANTIBODY ADHESION ONTO PROTEIN-ANTI-PROTEIN ANTIBODY COMPLEX;365
10.7.3;INFLUENCE OF TEMPERATURE ON THE NONSPECIFIC ANTIBODY ADHESION;365
10.7.4;NONSPECIFIC IMMUNOGLOBULIN ADHESION ONTO CARDIOLIPIN-LECITHIN ANTIGEN-SYPHILITIC REAGIN COMPLEX;366
10.7.5;NONSPECIFIC IMMUNOGLOBULIN ADHESION ONTO THE COMPLEX OF ETHYL CHLOROFORMATE- POLYMERIZED HUMAN .G AND PURIFIED ANTI-HUMAN .G;366
10.7.6;REFERENCES;367
10.8;Chapter 57. Evidence and Preliminary Study of a Complex Formed Between Trypsin and Rabbit a2 Macroglobulin;368
10.8.1;INTRODUCTION;368
10.8.2;METHODS;368
10.8.3;RESULTS;368
10.8.4;DISCUSSION;372
10.8.5;REFERENCES;372
10.9;Chapter 58. Dissociation Studies on a Naturally Occurring 22S Rheumatoid Factor;374
10.9.1;METHODS;374
10.9.2;RESULTS;374
10.9.3;DISCUSSION;376
10.9.4;SUMMARY;377
10.9.5;ACKNOWLEDGEMENTS;377
10.9.6;REFERENCES;377
10.10;Chapter 59. Isolation of a 6S Fragment by Proteolytic Digestion of Aggregated Human IgG;378
10.10.1;REFERENCES;382
10.11;Chapter 60. Theoretical and Practical Aspects of the Differences Between the lodometrical and Immunological Estimation of Serum Haptoglobin;384
10.11.1;REFERENCES;387
10.12;Chapter 61. Isolation of the Molecular Variants of Haptoglobin from Rat Serum;388
10.12.1;MATERIALS AND METHODS;388
10.12.2;RESULTS;390
10.12.3;DISCUSSION;391
10.12.4;REFERENCES;393
10.13;Chapter 62. Haptoglobin Porto Alegre;396
10.13.1;MATERIAL AND METHODS;396
10.13.2;RESULTS AND DISCUSSION;396
10.13.3;REFERENCES;399
10.14;Chapter 63. Evidence of a Plasmatic Factor Inhibiting the Haptoglobin-Hemoglobin Binding at the Onset of an Inflammatory Reaction;400
10.14.1;PRESENCE OF Hp IN RAT PLASMAS AT THE ONSET OF AN INFLAMMATORY REACTION ALTHOUGH THE Hp INDEX IS EQUAL TO ZERO;400
10.14.2;INHIBITION OF THE BINDING OF HAPTOGLOBIN WITH HEMOGLOBIN: FORMATION OF THE Hp-Hb COMPLEX AFTER DEAE-CELLULOSE CHROMATOGRAPHY OF A PLASMA WHOSE Hp INDEX EQUALS ZERO;401
10.14.3;EVIDENCE OF A PLASMATIC INHIBITOR PREVENTING THE HAPTOGLOBIN BINDING WITH HEMOGLOBIN AT THE ONSET OF AN INFLAMMATORY REACTION;401
10.14.4;SUMMARY;402
10.14.5;REFERENCES;402
11;SECTION D: Techniques;404
11.1;Chapter 64. Chemical Fixation of Proteins to Water-Insoluble Carriers;406
11.1.1;MATERIALS AND METHODS;407
11.1.2;RESULTS;407
11.1.3;ACKNOWLEDGEMENT;412
11.1.4;REFERENCES;412
11.2;Chapter 65. Detection of Proteins with Chemically Modified Bacteriophages;414
11.2.1;DETECTION OF ANTI-PROTEIN ANTIBODIES;414
11.2.2;DETECTION AND QUANTITATION OF PROTEINS;418
11.2.3;COMPARISON OF LYSOZYMES AND THEIR "LOOP" REGIONS;420
11.2.4;CONCLUDING REMARKS;421
11.2.5;REFERENCES;422
11.3;Chapter 66. Biospecific Adsorbents Based on Enzyme-Inhibitor Interaction;424
11.3.1;INTRODUCTION;424
11.3.2;ADSORPTION AND DESORPTI0N OF TRYPSIN AND CHYM0TRYPSIN ON INHIBITOR-AGAROSE;426
11.3.3;REFERENCES;430
11.4;Chapter 67. Preparation of High Capacity Immunosorbents with EMA;432
11.4.1;SUMMARY;436
11.4.2;REFERENCES;437
11.5;Chapter 68. Immunological Studies Using Insoluble Protein Polymers;438
11.5.1;IMMUNOLOGICALLY COATED PARTICLES;438
11.5.2;ANTIGEN-ACTIVE POLYMER PARTICLES;442
11.5.3;ROSETTE AND BLAST-TRANSFORMATION;444
11.5.4;PROTEIN POLYMER FILMS;444
11.5.5;GENERAL DISCUSSION;445
11.5.6;REFERENCES;446
11.6;Chapter 69. Elaboration of Transport Regulation Models Using Reticulated Enzyme-Bearing Membranes;448
11.6.1;I—PROPERTIES OF ENZYMES AFTER BONDING;448
11.6.2;II—MODELS OF TRANSPORT AND REGULATION;449
11.6.3;REFERENCES;453
11.7;Chapter 70. Protein-Bactenophage Conjugation, Bromacetyl Cellulose and Polyacrylamide Gel in the Study of Antibodies in Young and Adult Monkeys;454
11.7.1;REFERENCES;457
11.8;Chapter 71. Purification of Functional Immunoglobulins of the Rat by Preparative Methods and Immunoabsorbents;458
11.8.1;RESULTS;459
11.8.2;DISCUSSION;461
11.8.3;SUMMARY;461
11.8.4;REFERENCES;461
11.9;Chapter 72. Sepharose-Bound Chymotrypsin in Column Experiments;462
11.9.1;SUMMARY;467
11.9.2;REFERENCES;467
11.10;Chapter 73. Thin Layer Ultracentrifugation;468
11.10.1;SINGLE CAVITY ROTOR;468
11.10.2;REFERENCE;477
11.11;Chapter 74. Adsorption-Elution and Gel Exclusion Chromatography on Chromium Tanned Gelatin;478
11.11.1;REFERENCES;481
11.12;Chapter 75. Polarography of Proteins and the Changes of Denaturability of Human Albumin in Health and Disease;482
11.12.1;REFERENCES;487
11.13;Chapter 76. An Improved Method of Insulin Determination in Blood by the Application of Cellulose Adsorption Technique;488
11.13.1;SUMMARY;491
11.13.2;REFERENCES;491
11.14;Chapter 77. Poly acrylamide Electrophoresis: A Preparative Technique and its Application in Complement Chemistry;492
11.14.1;ACKNOWLEDGEMENT;495
11.14.2;REFERENCES;495
11.15;Chapter 78. A New Method of Primary Specific Colouring for Histological Sections: Addition of Competitively Binding Colloids to a Dye Solution;496
11.15.1;ACKNOWLEDGEMENT;498
11.15.2;REFERENCES;498
11.16;Chapter 79. Filtration of Macroglobulins from Human Serum Through Porous Glass;500
11.16.1;REFERENCES;502
11.17;Chapter 80. Lipoprotein Variants in Disc Electrophoresis—The El(C)* System'];504
11.17.1;METHODS;504
11.17.2;RESULTS AND DISCUSSION;506
11.17.3;ACKNOWLEDGEMENTS;509
11.17.4;REFERENCES;509
11.18;Chapter 81. Analytical and Preparative Isoelectric Focusing of Proteins in Sephadex and Bio-Gel Layers;510
11.18.1;REFERENCES;514
11.19;Chapter 82. Isoelectric Fractionation of Horseradish Peroxidase;516
11.19.1;REFERENCES;520
11.20;Chapter 83. The Amino Acid Composition of Proteins Isolated by Isoelectric Focusing;522
11.20.1;INTRODUCTION;522
11.20.2;EXPERIMENTAL;522
11.20.3;RESULTS;523
11.20.4;DISCUSSION;525
11.20.5;REFERENCES;525
11.21;Chapter 84. The Contribution of Bound Fatty Acids to the Heterogeneity of BSA;526
11.21.1;MATERIALS AND METHODS;526
11.21.2;RESULTS;529
11.21.3;DISCUSSION;530
11.21.4;REFERENCES;530
11.22;Chapter 85. Multiple Forms of Acid Phosphatase from Human Prostate Gland;532
11.22.1;REFERENCES;536
11.23;Chapter 86. Isoelectric Focusing of a1 Acute Phase Globulins of Rat Plasma;538
11.23.1;RESULTS;538
11.23.2;DISCUSSION;540
11.23.3;REFERENCES;541
11.24;Chapter 87. New Methods for Determination of Insulin-Binding Proteins by Immunoelectrophoresis;542
11.24.1;REFERENCES;545
11.25;Chapter 88. Determination of the Absolute Antibody Concentration in Precipitating Antisera Using Enzymatic Digestion of Immunoprecipitates;546
11.25.1;INTRODUCTION;546
11.25.2;MATERIAL AND METHODS;546
11.25.3;SUMMARY;554
11.25.4;REFERENCES;554
11.26;Chapter 89. Subclasses in Chromatographically Isolated IgG Preparations;556
11.26.1;MATERIAL AND METHODS;556
11.26.2;RESULTS AND DISCUSSION;557
11.26.3;SUMMARY;559
11.26.4;ACKNOWLEDGEMENTS;560
11.26.5;REFERENCES;560
11.27;Chapter 90. Comparison of Quantitative Immunoelectrophoretic Patterns of Serum and Other Biological Fluids;562
11.27.1;MATERIAL AND METHODS;562
11.27.2;RESULTS;562
11.27.3;DISCUSSION;564
11.27.4;REFERENCES;564
11.28;Chapter 91. Characterization of Nucleotidyltransferases by Immunochemical Techniques;566
11.28.1;MATERIAL AND METHODS;566
11.28.2;RESULTS;568
11.28.3;COMMENTS;569
11.28.4;REFERENCES;569
11.29;Chapter 92. Immunochemical Studies of Nucleoside Tri- and Diphosphatases and of 5'-Nucleotidase Activity in Rat Liver Plasma Membranes;570
11.29.1;CONCLUSIONS;575
11.29.2;ACKNOWLEDGEMENT;576
11.29.3;REFERENCES;576
11.30;Chapter 93. Lipid and Esterase Reactions of the Duck Serum HDL Lipoproteins, the Apoprotein and their Immunoprecipitates;578
11.30.1;RESULTS;578
11.30.2;DISCUSSION;580
11.30.3;REFERENCES;582
11.31;Chapter 94. Cholestasis Abnormal Serum Lipoproteins;584
11.31.1;REFERENCES;587
11.32;Chapter 95. Heat Induced Changes in Human Plasma Lipoproteins;588
11.32.1;MATERIALS AND METHODS;588
11.32.2;RESULTS;589
11.32.3;CONCLUSION;592
11.32.4;REFERENCES;592
12;Author Index;594
13;Subject Index;596