Peeters Protides of the Biological Fluids

Proceedings of the Nineteenth Colloquium, Bruges, 1971
1. Auflage 2016
ISBN: 978-1-4831-4634-8
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 606 Seiten, Web PDF

Protides of the Biological Fluids is a compendium of papers presented at the XIX Colloquium held at Bruges in 1971. It focuses on three main topics: lipoproteins, proteins, and protein catabolism. The main section of this book contains 60 papers discussing the composition, structure, synthesis, genetics, and function of lipoproteins. Another section is devoted to dielectric relaxation, fluorescence depolarization and determination of protein structure. The final section discusses protein catabolism and the applications of immunoelectrophoresis to protein quantitation. Students and scientists looking for an extensive reference on protein chemistry will find this book invaluable.

Autoren/Hrsg.

Peeters, H.

Inhaltsverzeichnis

1;Front Cover;1
2;Protides of the Biological Fluids;4
3;Copyright Page ;5
4;Table of Contents;6
5;Preface;14
6;Acknowledgements;16
7;ACADEMIC LECTURE;18
8;Chapter 1. Immunochemical Studies on Human Gastro-intestinal Tumors;20
8.1;REFERENCES;22
9;SECTION A: Lipoproteins;24
9.1;Part A: Introduction;26
9.1.1;Chapter 2. Conceptual Development of the Classification Systems of Plasma Lipoproteins;26
9.1.1.1;OPERATIONAL CLASSIFICATION SYSTEMS OF PLASMA LIPOPROTEINS;26
9.1.1.2;CHEMICAL CLASSIFICATION SYSTEM OF PLASMA LIPOPROTEINS;29
9.1.1.3;ACKNOWLEDGEMENTS;35
9.1.1.4;REFERENCES;35
9.2;Part A1: Composition and Structure;38
9.2.1;Chapter 3. Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions and Divalent Cations;38
9.2.1.1;FRACTIONATION OF SERUM LIPOPROTEIN BY POLYANIONS;38
9.2.1.2;ABNORMAL LIPOPROTEIN OF JAUNDICED SERUM—NATURE OF THE INTERACTION LIPOPROTEIN-POLYANION-CATION;41
9.2.1.3;ISOLATION OF LIPOPROTEINS FROM HUMAN SERUM'3';42
9.2.1.4;ISOLATION OF LDL PLUS VLDL AND HDL BY PRECIPITATION WITH DEXTRAN SULFATE AND MnCl2 (3);43
9.2.1.5;ANALYTICAL STUDIES;44
9.2.1.6;SUMMARY;44
9.2.1.7;REFERENCES;44
9.2.2;Chapter 4. A Comparison between HDL Lipoproteins and Apoprotein- Phosphatidylcholine Cenapses;46
9.2.2.1;INTRODUCTION;46
9.2.2.2;MATERIALS AND METHODS;46
9.2.2.3;RESULTS;47
9.2.2.4;DISCUSSION;49
9.2.2.5;REFERENCES;50
9.2.3;Chapter 5. Comparative Studies on Different Lipid-staining Procedures, Especially for Cellulose Acetate Electrophoresis;52
9.2.3.1;INTRODUCTION;52
9.2.3.2;THE CONVENTIONAL FAT-SOLUBLE DYE-STAINING METHODS;52
9.2.3.3;OZONE-SCHIFF STAINING METHOD;53
9.2.3.4;CONCLUSION;56
9.2.3.5;REFERENCES;57
9.2.4;Chapter 6. Characteristics of Six Fractions Obtained from Human High Density Lipoprotein;58
9.2.4.1;ACKNOWLEDGEMENTS;62
9.2.4.2;REFERENCES;62
9.2.5;Chapter 7. Electrofocusing of Normal Apolipoproteins and the Amino Acids of Their Subfractions;64
9.2.5.1;INTRODUCTION;64
9.2.5.2;MATERIALS AND METHODS;64
9.2.5.3;RESULTS;65
9.2.5.4;DISCUSSION;69
9.2.5.5;ACKNOWLEDGEMENT;69
9.2.5.6;REFERENCES;70
9.2.6;Chapter 8. Studies on the "Native" and the in Vitro-obtained Apoprotein of Duck Serum HDL;72
9.2.6.1;REFERENCES;75
9.2.7;Chapter 9. Identification of Lipoprotein Families in High Density Lipoproteins of Normal Human Plasma;76
9.2.7.1;SUMMARY;79
9.2.7.2;REFERENCES;80
9.2.8;Chapter 10. The Use of Streptolysin O as a Macromolecular Marker of Free Cholesterol in the ß-Lipoprotein Molecule;82
9.2.8.1;RESULTS;84
9.2.8.2;ACKNOWLEDGEMENT;85
9.2.8.3;REFERENCES;86
9.2.9;Chapter 11. Salting-in Gel Chromatographic Separation of Serum Low Density Lipoproteins on Sephadex Columns;88
9.2.9.1;MATERIALS AND METHODS;88
9.2.9.2;RESULTS AND DISCUSSION;89
9.2.9.3;REFERENCES;91
9.2.10;Chapter 12. Lipid Composition of Lipoprotein Fractions;92
9.2.10.1;REFERENCES;96
9.2.11;Chapter 13. Studies on the Proteins of Cat Lymph Chylomicrons;98
9.2.11.1;REFERENCES;101
9.2.12;Chapter 14. Role of Serum Lipoproteins in Hemolysis Induced by Vipera aspis Venom Phospholipases;102
9.2.12.1;MATERIALS AND METHODS;102
9.2.12.2;RESULTS;102
9.2.12.3;SUMMARY;107
9.2.12.4;REFERENCES;107
9.2.13;Chapter 15. Agarose Gel, Cellulose Acetate, and Polyacrylamide Gel Lipoprotein Electrophoresis—A Comparison of Methods;108
9.2.13.1;METHODS;108
9.2.13.2;RESULTS;109
9.2.13.3;SUMMARY AND CONCLUSIONS;111
9.2.13.4;ACKNOWLEDGEMENTS;111
9.2.13.5;REFERENCES;111
9.2.14;Chapter 16. Staining of Lipoproteins in Cellulose Acetate Membrane;114
9.2.14.1;TECHNICAL DETAILS;116
9.2.14.2;REFERENCE;116
9.2.15;Chapter 17. Oxygenated Sterol Esters of Human Atherosclerotic Plaques;118
9.2.15.1;INTRODUCTION;118
9.2.15.2;METHODS;118
9.2.15.3;RESULTS;118
9.2.15.4;DISCUSSION;123
9.2.15.5;ACKNOWLEDGEMENTS;123
9.2.15.6;REFERENCES;123
9.3;Part A2: Synthesis and Degradation;126
9.3.1;Chapter 18. Comparative Studies on the in vitro Effects of Delipidation with Organic Solvents, Enzymatic Lipolysis, Action of 'Anionic, Non-ionic and Cationic Detergents and the in vivo Effect of Lipoproteinlipase on Duck Serum a-Lipoprotein;126
9.3.1.1;REFERENCES;133
9.3.2;Chapter 19. Study of the Human Serum ß-Lipoprotein Antigenicity according to Its Degree of Delipidation;134
9.3.2.1;DELIPIDATION METHODS;134
9.3.2.2;RESULTS;135
9.3.2.3;DISCUSSION;136
9.3.2.4;CONCLUSION;137
9.3.2.5;REFERENCES;137
9.3.3;Chapter 20. Influence of Cholesterol on the Concentration, Composition and Synthesis of Chicken Serum Lipoproteins;138
9.3.3.1;SUMMARY;142
9.3.3.2;REFERENCES;142
9.3.4;Chapter 21. Isovaleric Acid in Acoustic Tissues of Porpoises: Triacylglycerols Resistant to Porcine Pancreatic Lipase;144
9.3.4.1;ACKNOWLEDGEMENT;146
9.3.4.2;REFERENCES;146
9.3.5;Chapter 22. Effect of Clofibrate on Lipoprotein Metabolism;148
9.3.5.1;ACKNOWLEDGEMENTS;151
9.3.5.2;REFERENCES;151
9.3.6;Chapter 23. Biosynthesis of Plasma Glycolipoproteins;152
9.3.6.1;GLYCOSYLATION OF PLASMA LIPOPROTEINS;152
9.3.6.2;EFFECT OF CDP-CHOLINE ON N-ACETYLGLUCOSAMINYL TRANSFERASE IN VARIOUS SUB-CELLULAR FRACTIONS;153
9.3.6.3;EFFECT OF DIFFERENT LIPID FACTORS ON N-ACETYLGLUCOSAMINYL TRANSFERASE ENZYME;153
9.3.6.4;MECHANISM OF CDP-CHOLINE ACTION;154
9.3.6.5;EFFECT OF CDP-CHOLINE ON GLYCOSYLATION OF THE j8-LIPOPROTEINS;155
9.3.6.6;ACKNOWLEDGEMENTS;155
9.3.6.7;REFERENCES;155
9.3.7;Chapter 24. Human Intestinal Lipoprotein Particle Synthesis and Transport;158
9.3.7.1;SUMMARY;162
9.3.7.2;REFERENCES;164
9.3.8;Chapter 25. The Metabolism of the Plasma Cholesterol in Man;166
9.3.8.1;WHOLE-BODY EXCHANGEABLE CHOLESTEROL;166
9.3.8.2;PLASMA CHOLESTERYL ESTERS;166
9.3.8.3;TURNOVER OF THE EXCHANGEABLE MASS OF CHOLESTEROL;168
9.3.8.4;CHANGES IN TURNOVER OF THE PLASMA CHOLESTEROL;170
9.3.8.5;REFERENCES;170
9.3.9;Chapter 26. Metabolism of Very Low Density Lipoproteins in Diabetes Mellitus;172
9.3.9.1;REFERENCES;173
9.4;Part A3: Genetics;174
9.4.1;Chapter 27. The Ag-System—Present Concepts and Immunogenetic Models;174
9.4.1.1;REFERENCES;177
9.4.2;Chapter 28. Serology and Genetics of the Ag-System;178
9.4.2.1;REMARKS ON Ag-TYPING PROCEDURES;178
9.4.2.2;GENETICS OF THE Ag-SYSTEM;179
9.4.2.3;REMARKS ON Ag-ANTIBODIES;182
9.4.2.4;REFERENCES;183
9.4.3;Chapter 29. Genetic Polymorphisms of Lipoproteins;186
9.4.3.1;CONCERNING THE IMMUNOLOGY OF THE Lp SYSTEM;187
9.4.3.2;INTERPRETATIONAL PROBLEMS IN THE Lp SYSTEM;187
9.4.3.3;SOME DIFFICULTIES AND PARADOXES ENCOUNTERED IN WORK WITH THE Lp AND Ag SYSTEMS;189
9.4.3.4;SERUM LIPOPROTEINS AND CELL MEMBRANES;189
9.4.3.5;REFERENCES;194
9.4.4;Chapter 30. Immunological Relationship and Chemical Similarities between HDL Lipoproteins from Various Mammalian and Avian Species;196
9.4.4.1;REFERENCES;198
9.4.5;Chapter 31. On the Significance of the El* (BA) Variants;200
9.4.5.1;METHODS;200
9.4.5.2;RESULTS;202
9.4.5.3;DISCUSSION;206
9.4.5.4;REFERENCES;206
9.4.6;Chapter 32. Subunit Structure of the Lp{d) Lipoprotein;208
9.4.6.1;REFERENCES;212
9.4.7;Chapter 33. The Isolation and Partial Purification of Low Density Lipoproteins Carrying the Ag(x) and Ag(y) Antigenic Determinants;214
9.4.7.1;MATERIALS AND METHODS;214
9.4.7.2;RESULTS;215
9.4.7.3;ACKNOWLEDGEMENTS;217
9.4.7.4;REFERENCES;217
9.4.8;Chapter 34. A Serum Type of Isoprecipitin System of HD Lipoproteins;218
9.4.8.1;MATERIALS AND METHODS;218
9.4.8.2;RESULTS AND DISCUSSION;218
9.4.8.3;ACKNOWLEDGEMENT;220
9.4.8.4;REFERENCES;220
9.4.9;Chapter 35. Plasma Lipoprotein Distribution in Normal Children and Adults of Different Ages;222
9.4.9.1;INTRODUCTION;222
9.4.9.2;MATERIALS AND METHODS;222
9.4.9.3;RESULTS;222
9.4.9.4;DISCUSSION;225
9.4.9.5;REFERENCES;225
9.5;Part A4: Function;226
9.5.1;Chapter 36. The Arterial Foam Cell—in vitro and in vivo Studies;226
9.5.1.1;REFERENCES;231
9.5.2;Chapter 37. A New Method of Preparation for the Apoprotein Moiety from Folch-Pi Proteolipid;234
9.5.2.1;APOPROTEIN PREPARATION;234
9.5.2.2;COMPARATIVE STUDY OF THE APOPROTEINS;235
9.5.2.3;DISCUSSION;236
9.5.2.4;REFERENCES;237
9.5.3;Chapter 38. Evidence for a Covalent Bond between Lipid and a Membrane Protein {Rigid Layer of the Cell Wall ofE. coli);238
9.5.3.1;REFERENCES;241
9.5.4;Chapter 39. Distribution of Lipoproteins in Arteries and Tissue as Determined by Immunohistological and Radioactive Tracer Methods;242
9.5.4.1;ARTERIAL LESIONS (Atherosclerosis);242
9.5.4.2;XANTHOMATA;242
9.5.4.3;CORNEAL ARCUS FORMATION;243
9.5.4.4;DISCUSSION;243
9.5.4.5;REFERENCES;243
9.5.5;Chapter 40. Mechanism of Uptake of Plasma Lipoprotein Triglyceride by Avian Ovarian Follicles;244
9.5.5.1;IN VITRO SYSTEM TO STUDY LIPOPROTEIN LIPID UPTAKE BY AVIAN OOCYTES;244
9.5.5.2;IN VITRO UPTAKE OF VLDL TRIGLYCERIDE;245
9.5.5.3;EFFECT OF PROTAMINE-SULFATE AND HEPARIN ON VLDL TRIGLYCERIDE UPTAKE;246
9.5.5.4;UPTAKE OF DOUBLE-LABELED VLDL TRIGLYCERIDE;248
9.5.5.5;REFERENCES;248
9.5.6;Chapter 41. Uptake of Plasma Lipoproteins by the Mammary Gland ofLactating Cows;250
9.5.6.1;REFERENCES;253
9.5.7;Chapter 42. Cholesterol Movements between Rat Plasmatic Lipoproteins and Red Cells in vitro;254
9.5.7.1;MATERIALS AND METHODS;254
9.5.7.2;RESULTS;254
9.5.7.3;DISCUSSION;256
9.5.7.4;REFERENCES;257
9.5.8;Chapter 43. The Formation of Lipoproteins in Hen Liver and Egg Yolk;260
9.5.8.1;REFERENCES;264
9.6;Part A5: Pathology;266
9.6.1;Chapter 44. Isolation and Properties of Abnormal Serum Lipoproteins in Cholestasis;266
9.6.1.1;FRACTIONATION OF LOW DENSITY LIPOPROTEINS;266
9.6.1.2;IMMUNOLOGICAL PROPERTIES OF ABNORMAL LIPOPROTEINS;266
9.6.1.3;ABNORMAL LIPOPROTEIN PHOSPHOLIPIDS;269
9.6.1.4;REFERENCES;270
9.6.2;Chapter 45. Clinical Lipid Evaluations;272
9.6.2.1;SUMMARY AND CONCLUSIONS;277
9.6.2.2;ACKNOWLEDGEMENTS;277
9.6.2.3;REFERENCES;277
9.6.3;Chapter 46. Diagnosis of Familial Hypercholesterolaemia in Infancy;278
9.6.3.1;PATIENTS AND METHODS;278
9.6.3.2;RESULTS;278
9.6.3.3;CONCLUSION;281
9.6.3.4;ACKNOWLEDGEMENTS;281
9.6.3.5;REFERENCES;281
9.6.4;Chapter 47. Dyslipidaemias in the Neonatal Period;282
9.6.4.1;INTRODUCTION;282
9.6.4.2;MATERIALS AND METHODS;282
9.6.4.3;RESULTS;283
9.6.4.4;DISCUSSION OF THE CORD BLOOD RESULTS;287
9.6.4.5;SUMMARY;288
9.6.4.6;ACKNOWLEDGEMENTS;288
9.6.4.7;REFERENCES;288
9.6.5;Chapter 48. Human Myeloma Proteins which Bind ß-Lipoproteins;290
9.6.5.1;INTRODUCTION;290
9.6.5.2;SCREENING FOR BINDING ACTIVITY AGAINST LIPOPROTEINS;290
9.6.5.3;CORRELATION BETWEEN BINDING ACTIVITY AND SERUM LIPID CONCENTRATION;291
9.6.5.4;CHARACTERIZATION AND STUDIES ON THE SPECIFICITY OF AN IgA MYELOMA PROTEIN;292
9.6.5.5;RELATION BETWEEN MYELOMA PROTEIN AND LIPID CONCENTRATION IN SERUM (L.W.);292
9.6.5.6;DISCUSSION;294
9.6.5.7;ACKNOWLEDGEMENTS;295
9.6.5.8;REFERENCES;295
9.6.6;Chapter 49. Primary Hyperlipoproteinaemias in Myocardial Infarction;296
9.6.6.1;REFERENCES;299
9.6.7;Chapter 50. Type III Hyperlipoproteinaemia with Xanthomatosis;300
9.6.7.1;METHODS;300
9.6.7.2;RESULTS;301
9.6.7.3;REFERENCES;304
9.6.8;Chapter 51. A Simple Method for the Detection and Identification of Various Types of Hyperlipoproteinemia;306
9.6.8.1;RESULTS;307
9.6.8.2;SUMMARY;309
9.6.8.3;REFERENCES;309
9.6.9;Chapter 52. Problem Cases in Phenotyping;310
9.6.9.1;THE BASIS OF PHENOTYPING;310
9.6.9.2;RANGES OF THE DISCRIMINATING PARAMETERS;310
9.6.9.3;SHIFTS IN PHENOTYPE;312
9.6.9.4;BORDERLINE PATHOLOGY;312
9.6.10;Chapter 53. Phenotyping of Hyperlipidaemias by Electrochromatography;316
9.6.10.1;INTRODUCTION;316
9.6.10.2;MATERIAL AND METHODS;316
9.6.10.3;RESULTS;317
9.6.10.4;DISCUSSION;321
9.6.10.5;ACKNOWLEDGEMENT;322
9.6.10.6;REFERENCES;322
9.6.11;Chapter 54. Lipid Electrophoresis on Cellulose Acetate Membranes and on Agarose;324
9.6.11.1;SUMMARY;327
9.6.11.2;REFERENCES;327
9.6.12;Chapter 55. Quantitative Lipoprotein andLipid Determinations in Patients with Various Malignancies;328
9.6.12.1;INTRODUCTION;328
9.6.12.2;MATERIAL AND METHODS;328
9.6.12.3;RESULTS;328
9.6.12.4;DISCUSSION;331
9.6.12.5;REFERENCES;331
9.6.13;Chapter 56. A General Review of Clinical and Laboratory Features of Familial Hypercholesterolemia (Type II Hyperbetalipoproteinemia);332
9.6.13.1;THE HOMOZYGOTES;332
9.6.13.2;THE HETEROZYGOTES;334
9.6.13.3;GENETICS;334
9.6.13.4;DISCUSSION;335
9.6.13.5;ACKNOWLEDGEMENT;335
9.6.13.6;REFERENCES;335
9.7;Part A6: Dietary Measures and Drug Therapy;336
9.7.1;Chapter 57. The Validity of Animal Models in the Study of Atherogenesis;336
9.7.1.1;INTRODUCTION;336
9.7.1.2;THE SELECTION OF A SUITABLE ANIMAL MODEL;336
9.7.1.3;A BRIEF REVIEW OF EXPERIMENTALLY INDUCED ATHEROSCLEROSIS;337
9.7.2;Chapter 58. Dietary and Drug Management of Familial Hypercholesterolemia;340
9.7.2.1;RESULTS;340
9.7.2.2;DISCUSSION;343
9.7.2.3;ACKNOWLEDGEMENT;343
9.7.2.4;REFERENCES;343
9.7.3;Chapter 59. Oxidative Metabolism in Normal and Hyperlipemic Patients;344
9.7.3.1;MODEL;344
9.7.3.2;CALCULATIONS ASSOCIATED WITH THE MODEL;345
9.7.3.3;REFERENCES;346
9.7.4;Chapter 60. Management of Abetalipoproteinaemia in Childhood;348
9.7.4.1;CONTROL OF MALABSORPTION AND MAINTENANCE OF GROWTH;348
9.7.4.2;VITAMIN SUPPLEMENTATION AND POSSIBLE PREVENTION OF NEUROLOGICAL AND RETINAL LESIONS;348
9.7.4.3;ACKNOWLEDGEMENTS;352
9.7.4.4;REFERENCES;352
9.7.5;Chapter 61. Dietary and Hormonal Modifications of Rat Serum Lipoproteins;354
9.7.5.1;REFERENCES;356
9.7.6;Chapter 62. The Lipoproteins in Hyperlipidaemic Primates as a Model for Human Atherosclerosis;358
9.7.6.1;LIPOPROTEINS IN BABOONS GIVEN AN ATHEROGENIC DIET;358
9.7.6.2;TYPE II HYPERLIPOPROTEINAEMIA;358
9.7.6.3;COMPARISON BETWEEN MAN, BABOON AND CHIMPANZEE;359
9.7.6.4;CONCLUSIONS;360
9.7.6.5;REFERENCES;360
10;SECTION B: Hydrodynamic Properties of Proteins;362
10.1;Part B1: Dielectrics;364
10.1.1;Chapter 63. The Role of Dielectric Studies in the Investigation of Protein Molecules;364
10.1.1.1;ABSTRACT;364
10.1.1.2;INTRODUCTION;364
10.1.1.3;DISCUSSION;365
10.1.1.4;GENERAL CONCLUSIONS;369
10.1.1.5;REFERENCES;369
10.1.2;Chapter 64. The Dipole Moment of Urea and Thiourea in Water and Ethane Diol;372
10.1.2.1;RESULTS AND DISCUSSION;372
10.1.2.2;REFERENCES;374
10.1.3;Chapter 65. Measurement of the Hydration of Biological Molecules from Dielectric Measurements at Centimetre Wavelengths;376
10.1.3.1;INTRODUCTION;376
10.1.3.2;METHODS AND MATERIALS;376
10.1.3.3;RESULTS;377
10.1.3.4;DISCUSSION;378
10.1.3.5;CONCLUSIONS;383
10.1.3.6;ACKNOWLEDGEMENT;383
10.1.3.7;REFERENCES;383
10.1.4;Chapter 66. Recent Progress in the Dielectric Research on Polynucleotides;384
10.1.4.1;DIELECTRIC STUDIES OF DNA IN SHEAR GRADIENT;384
10.1.4.2;DIELECTRIC RELAXATION OF SYNTHETIC POLYNUCLEOTIDES;386
10.1.4.3;ACKNOWLEDGEMENT;387
10.1.4.4;REFERENCES;387
10.1.5;Chapter 67. Permittivity Measurements on Biological Solutions at Very High Frequency;388
10.1.5.1;INTRODUCTION;388
10.1.5.2;THEORETICAL;388
10.1.5.3;EXPERIMENTAL;390
10.1.5.4;RESULTS;392
10.1.5.5;CONCLUSION;393
10.1.5.6;REFERENCES;393
10.1.6;Chapter 68. Deduction of Size and Hydration of Protein Molecules from Permittivity Measurements;394
10.1.6.1;INTRODUCTION;394
10.1.6.2;THEORETICAL;394
10.1.6.3;MATERIALS AND METHODS;395
10.1.6.4;RESULTS;395
10.1.6.5;CONCLUSION;398
10.1.6.6;REFERENCES;399
10.2;Part B2: Fluorescence;400
10.2.1;Chapter 69. The Use of Fluorescence Techniques for Studying the Structure and Structural Transitions of Polypeptides in Solution;400
10.2.1.1;TECHNIQUE;400
10.2.1.2;UREA EFFECTS;401
10.2.1.3;MOLECULAR TRANSITIONS;402
10.2.1.4;SUMMARY;409
10.2.1.5;REFERENCES;409
10.2.2;Chapter 70. The Decay of the Fluorescence Anisotropy of Biological Macromolecules;410
10.2.2.1;INTRODUCTION;410
10.2.2.2;THEORETICAL ASPECT;410
10.2.2.3;EXPERIMENTAL DETAILS;412
10.2.2.4;APPLICATION OF THE METHOD;412
10.2.2.5;REFERENCES;414
10.2.3;Chapter 71. The Influence of the Microenvironments of the Tryptophan Groups of Proteins upon Their Fluorescence Properties;416
10.2.3.1;REFERENCES;418
10.2.4;Chapter 72. Determination of Fluorescence Lifetimes;420
10.2.4.1;METHOD;420
10.2.4.2;RESULTS;421
10.2.4.3;REFERENCES;424
10.2.5;Chapter 73. Hydrodynamic Studies of Some Lysozymes and a-Lactalbumins;426
10.2.5.1;INTRODUCTION;426
10.2.5.2;EXPERIMENTAL PROCEDURE;426
10.2.5.3;RESULTS AND DISCUSSION;427
10.2.5.4;SUMMARY;428
10.2.5.5;ACKNOWLEDGEMENTS;428
10.2.5.6;REFERENCES;428
11;SECTION C: Protein Catabolism;430
11.1;Chapter 74. Catabolism and Distribution of IgG in Different Diseases;432
11.1.1;MATERIAL;432
11.1.2;METHODS;432
11.1.3;RESULTS;433
11.1.4;DISCUSSION;438
11.1.5;ACKNOWLEDGEMENT;440
11.1.6;REFERENCES;440
11.2;Chapter 75. Disorders of Immunoglobulin Catabolism;442
11.2.1;INTRODUCTION;442
11.2.2;ENDOGENOUS HYPERCATABOLISM AFFECTING A SINGLE PROTEIN;442
11.2.3;ENDOGENOUS HYPERCATABOLISM AFFECTING SEVERAL SERUM PROTEINS;443
11.2.4;SUMMARY;444
11.2.5;REFERENCES;444
11.3;Chapter 76. Thermodynamics of the Denaturation of Human Serum Albumin in Relation to Intra-vital Breakdown;446
11.3.1;INTRODUCTION;446
11.3.2;MATERIALS AND METHODS;448
11.3.3;RESULTS AND DISCUSSION;448
11.3.4;CONCLUSION;452
11.3.5;REFERENCES;453
11.4;Chapter 77. Plasma Protein Catabolism in the Capillary Wall?;454
11.4.1;INTRODUCTION;454
11.4.2;METHODS;455
11.4.3;RESULTS;455
11.4.4;DISCUSSION;455
11.4.5;SUMMARY;458
11.4.6;REFERENCES;458
11.5;Chapter 78. IgM Degradation in Ménétrier's Disease (Giant Hypertrophic Gastritis);460
11.5.1;METHODS;460
11.5.2;CASE MATERIAL;461
11.5.3;RESULTS AND DISCUSSION;461
11.5.4;REFERENCES;464
11.6;Chapter 79. IgA Metabolism in Control Subjects, in Patients with Elevated IgA Levels and in Patients with IgA Deficiency;466
11.6.1;INTRODUCTION;466
11.6.2;PATIENTS;466
11.6.3;METHODS;468
11.6.4;RESULTS;468
11.6.5;DISCUSSION;469
11.6.6;ACKNOWLEDGEMENT;469
11.6.7;REFERENCES;470
11.7;Chapter 80. Possible Enhancement oflgM Catabolism by D-Penicillamine;472
11.7.1;CASE SUMMARY;474
11.7.2;DISCUSSION;474
11.7.3;SUMMARY;476
11.7.4;REFERENCES;476
11.8;Chapter 81. The Catabolism of Human .G-Globulin and Its Fragments in Man and Mouse;478
11.8.1;METHODS;478
11.8.2;RESULTS;479
11.8.3;DISCUSSION;480
11.8.4;ACKNOWLEDGEMENTS;482
11.8.5;REFERENCES;482
11.9;Chapter 82. A Consideration of Short-term Measurement of Plasma Protein Catabolic Rates;484
11.9.1;CATABOLIC RATE MEASUREMENTS IN THIS STUDY;484
11.9.2;REFERENCES;488
11.10;Chapter 83. Rate of Synthesis of Albumin during the Early Postoperative Period;490
11.10.1;INTRODUCTION;490
11.10.2;PREOPERATIVE MODEL;490
11.10.3;POSTOPERATIVE MODEL;491
11.10.4;CONCLUSION;493
11.10.5;REFERENCES;493
11.11;Chapter 84. The Isolated Perfused Liver in the Study of Albumin Synthesis;494
11.11.1;DISCUSSION;497
11.11.2;ACKNOWLEDGEMENTS;498
11.11.3;REFERENCES;498
11.12;Chapter 85. Albumin Metabolism in Patients with Chronic Renal Failure on Low Protein Balanced Diet;500
11.12.1;INTRODUCTION;500
11.12.2;MATERIALS AND METHODS;500
11.12.3;RESULTS;501
11.12.4;CONCLUSIVE REMARKS;503
11.12.5;REFERENCES;504
11.13;Chapter 86. Regulation of Transferrin Synthesis;506
11.13.1;INTRODUCTION;506
11.13.2;METHODS;506
11.13.3;EXPERIMENTS AND RESULTS;507
11.13.4;DISCUSSION;509
11.13.5;ACKNOWLEDGEMENTS;510
11.13.6;REFERENCES;510
11.14;Chapter 87. Catabolism of Nonmitochondrial Glutamic Oxalacetic Transaminase from Pig Heart in Piglets;512
11.14.1;REFERENCES;516
11.15;Chapter 88. The Turnover of Lysozyme;518
11.15.1;MATERIALS AND METHODS;518
11.15.2;RESULTS AND DISCUSSION;518
11.15.3;REFERENCES;521
12;SECTION D: New Methods;522
12.1;Chapter 89. Studies of Protein Interactions by Microcalorimetry;524
12.1.1;MICROCALORIMETERS;525
12.1.2;THERMOCHEMICAL STUDIES OF PROTEIN BINDING REACTION;527
12.1.3;REFERENCES;529
12.2;Chapter 90. Isotachophoresis in Capillary Tubes;530
12.2.1;INTRODUCTION;530
12.2.2;SEPARATION PRINCIPLES;530
12.2.3;DETECTION METHODS;532
12.2.4;EXPERIMENTAL;533
12.2.5;SEPARATIONS;533
12.2.6;DISCUSSION;536
12.2.7;REFERENCES;536
12.3;Chapter 91. The Isolation of Human Transferrin by Isotachophoresis;538
12.3.1;INTRODUCTION;538
12.3.2;FRACTIONATION OF SERUM;538
12.3.3;RESULTS;540
12.3.4;CONCLUSIONS;540
12.3.5;ACKNOWLEDGEMENTS;541
12.3.6;REFERENCES;541
12.4;Chapter 92. Oil-cooled Apparatus for Quantitative Immunoelectrophoresis;542
12.4.1;EXPERIMENTAL;542
12.4.2;DISCUSSION;543
12.4.3;ACKNOWLEDGEMENTS;544
12.4.4;REFERENCES;544
12.5;Chapter 93. Quantitative Immunoelectrophoresis Applied to the Analysis of Elution Profiles in Column Chromatography;546
12.5.1;MATERIALS AND METHODS;546
12.5.2;COMBINATION OF LINE- AND CROSSED-IMMUNOELECTROPHORESIS;546
12.5.3;COMBINATION OF LINE- AND ROCKET IMMUNOELECTROPHORESIS;546
12.5.4;RESULTS;547
12.5.5;DISCUSSION;549
12.5.6;ACKNOWLEDGEMENTS;549
12.5.7;REFERENCES;549
12.6;Chapter 94. A Modified Radioimmunoassay for Quantitative Determination of IgG Subclasses in Man;550
12.6.1;MATERIALS AND METHODS;550
12.6.2;RESULTS AND DISCUSSION;552
12.6.3;ACKNOWLEDGEMENTS;555
12.6.4;REFERENCES;557
12.7;Chapter 95. Immunoselection Technique and Its Applications;558
12.7.1;SUMMARY;561
12.7.2;REFERENCES;562
12.8;Chapter 96. Storage of Human Serum for Immunochemical Determination of the Serum Proteins;564
12.8.1;INTRODUCTION;564
12.8.2;METHODS;564
12.8.3;RESULTS AND COMMENTS;564
12.8.4;CONCLUSION;569
12.8.5;REFERENCES;569
12.9;Chapter 97. Enzyme-linked Immunosorbent Assay, ELISA;570
12.9.1;QUANTITATIVE DETERMINATION OF RABBIT IgG;570
12.9.2;DETERMINATION OF ANTIBODIES IN SERUM;571
12.9.3;ACKNOWLEDGEMENTS;573
12.9.4;REFERENCES;573
12.10;Chapter 98. Determination of Insulin Binding to ß-Lipoprotein in Sera from Normals and Diabetics Applying Immunoelectrophoresis;574
12.10.1;CONCLUSIONS;577
12.10.2;REFERENCES;577
12.11;Chapter 99. Candida Precipitins Characterized by a Modified Antigen-Antibody Crossed Electrophoresis;578
12.11.1;INTRODUCTION;578
12.11.2;MATERIALS AND METHODS;578
12.11.3;RESULTS AND DISCUSSION;579
12.11.4;SUMMARY;581
12.11.5;ACKNOWLEDGEMENT;581
12.11.6;REFERENCES;581
12.12;Chapter 100. An Automated System of Two-dimensional Immunoelectrophoresis;582
12.12.1;TECHNIQUE;582
12.12.2;ACKNOWLEDGEMENT;585
12.12.3;REFERENCES;585
12.13;Chapter 101. Micro-polyacrylamide Flat Gel Electrophoresis: Advantages and Applications;586
12.13.1;INTRODUCTION;586
12.13.2;METHODS;586
12.13.3;RESULTS;588
12.13.4;DISCUSSION;589
12.13.5;ACKNOWLEDGEMENTS;590
12.13.6;REFERENCES;590
12.14;Chapter 102. Comparison of Slab and Cylinder Gel Focusing;592
12.14.1;ACKNOWLEDGEMENTS;595
12.14.2;REFERENCES;595
12.15;Chapter 103. Studies of Aggregates of lgG in Human Sera and Commercial .-Globulin Preparations with a New Immuno-gel Filtration Method;596
12.15.1;MATERIAL AND METHODS;596
12.15.2;RESULTS;596
12.15.3;DISCUSSION;601
12.15.4;SUMMARY;601
12.15.5;REFERENCES;601
13;Author Index;602
14;Subject Index;604