Potrykus / Spangenberg Gene Transfer to Plants

Erscheinungsjahr 2013
ISBN: 978-3-642-79247-2
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 362 Seiten, eBook

Reihe: Springer Lab Manual

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Potrykus, Ingo

Spangenberg, German

Inhaltsverzeichnis

I Agrobacterium tumefaciens-Mediated Gene Transfer.- 1 Transformation of Arabidopsis thaliana Using Root Explants.- 2 Seed Transformation in Arabidopsis thaliana.- 3 In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum-Infiltration.- 4 Agrobacterium-Mediated Transformation of Potato (Solanum tuberosum).- 5 Agrobacterium-Mediated Transformation of Rapeseed (Brassica napus).- II Agrobacterium rhizogenes-Mediated Gene Transfer.- 6 Agrobacterium rhizogenes-Mediated Transformation: Transformed Roots to Transformed Plants.- III Direct Gene Transfer to Protoplasts.- 7 Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants.- 8 Polyethylene Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants.- 9 Plastid Transformation by Polyethylene Glycol Treatment of Protoplasts and Regeneration of Transplastomic Tobacco Plants.- 10 Electroporation-Mediated GeneTransfer to Protoplasts and Regeneration of Transgenic Plants.- 11 Electroporation Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants.- 12 Regeneration of Transgenic Maize Plants from Embryogenic Protoplasts After Polyethylene Glycol-Mediated DNA Uptake.- 13 Gene Transfer to Protoplasts: Transient Gene Expression Analysis.- IV Biolistic Plant Transformation.- 14 Transient Gene Expression Studies with the Biolistic System.- 15 Maize Transformation by Microprojectile Bombardment of Immature Embryos.- 16 Biolistic Transformation of Microspore-Derived and Immature Zygotic Embryos and Regeneration of Fertile Transgenic Barley Plants.- 17 Transformation of Soybean (Glycine max) Through Electric Discharge Particle Acceleration.- 18 Ballistic Microtargeting of Visible Marker Genes to the Shoot Meristem of Wheat.- 19 Stable Transformation of Wheat by Microprojectile Bombardment.- 20 Biolistic-Based Analysis of Chloroplast Gene Expression.- V Microinjectionand Fiber-Mediated Transformation.- 21 Microinjection into Tobacco Protoplasts and Regeneration of Transgenic Plants.- 22 Silicon Carbide Whisker-Mediated Transformation: Regeneration of Transgenic Maize Plants.- VI Tissue Electroporation.- 23 Transient Expression Following Electroporation into Immature Zygotic Embryos of Wheat.- 24 Transgenic Indica Rice Plants by Electroporation of Seed Embryo Cells.- VII Analysis of Transgenic Plants.- 25 Standard Molecular Techniques for the Analysis of Transgenic Plants.- 26 In Situ Hybridization on Plant Metaphase Chromosomes I. Farbos and A. Mouras.- 27 Localisation of RNA Transcripts in Plant Tissue by in Situ Hybridisation.- 28 In Situ Immunolocalisation of Transgene Products in Transgenic Plants.- VIII Establishment and Maintenance of Embryogenic Cultures.- 29 Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and Their Protoplasts in Forage Grasses.- IX Genetic Markers and Expression Signals.- 30 Expression Signals and Vectors.- 31 Selectable Marker and Reporter Genes.- X Guidelines and Biosafety Considerations.- 32 Release of Transgenic Plants: Regulations and Developments.- 33 Risk Assessment.