Rampal Microarrays

Volume I: Synthesis Methods
2. Auflage 2007
ISBN: 978-1-59745-303-5
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 381, 452 Seiten, eBook

Reihe: Methods in Molecular Biology

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments.Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology.Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Zielgruppe

Research

Autoren/Hrsg.

Rampal, Jang B.

Inhaltsverzeichnis

Current Microarray Surface Chemistries.- Nonfouling Surfaces.- Optimization of Oligonucleotide DNA Microarrays.- Detection of DNA Copy Number Alterations in Complex Genomes Using Array Comparative Genomic Hybridization.- Evaluating the Quality of Data From Microarray Measurements.- Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents.- Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Preparation of Biochips.- Methods in High-Resolution, Array-Based Comparative Genomic Hybridization.- Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression Analysis.- Construction of In Situ Oligonucleotide Arrays on Plastic.- Detecting Ligated Fragments on Oligonucleotide Microarrays.- Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip.- Hybridization Analysis Using Oligonucleotide Probe Arrays.- In Situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing.- Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays.- Printing Low Density Protein Arrays in Microplates.- Forward-Phase and Reverse-Phase Protein Microarray.- Cell Microarray for Functional Exploration of Genomes.- Quantification of Mixed-Phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence.- Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays.- Nanotechnology.