Rao Sanadi Current Topics in Bioenergetics

Volume 9
1. Auflage 2014
ISBN: 978-1-4832-1693-5
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 436 Seiten, Web PDF

Current Topics in Bioenergetics, Volume 9 presents the theoretical, thermodynamic perspective of energy transducing reactions. This book provides a detailed kinetic analysis of a specific aspect of an ion pump. Organized into seven chapters, this volume begins with an overview of the quantitative relations between measurable parameters of energy-transducing systems. This text then examines the probes for intracellular pH determination, which stimulate the development of additional methods and their application in pathology and pharmacology. Other chapters consider studies with isolated proteins or protein complexes derived from the membranes. This book discusses as well the chemistry of photosynthesis and oxidative phosphorylation. The final chapter deals with the advances in the use of photo affinity labeling in the study of the structure of ligand sites on proteins, which became possible only in conjunction with the development of methods for the isolation of peptides and their sequence determination. This book is a valuable resource for biologists and biochemists.

Autoren/Hrsg.

Rao Sanadi, D.

Inhaltsverzeichnis

1;Front Cover;1
2;Current Topics in Bioenergetics;4
3;Copyright Page;5
4;Table of Contents;6
5;List of Contributors;10
6;Preface;12
7;Contents of Previous Volumes;14
8;Chapter 1. Irreversible Thermodynamic Description of Energy Transduction in Biomembranes;18
8.1;I. Introduction;19
8.2;II. From Theoretical Physics to Membrane Biochemistry;20
8.3;III. Development of the Description of a Biological System by Building from Thermodynamically Independent Units;29
8.4;IV. Oxidative Phosphorylation in Mitochondria;35
8.5;V. Light-Energized Systems;45
8.6;VI. Transport Across Plasma Membranes;55
8.7;VII. Intermediary Metabolism and Biomembranes;68
8.8;VIII. Conclusion;73
8.9;Acknowledgments;74
8.10;References;76
9;Chapter 2. Intracellular pH: Methods and Applications;80
9.1;I. Introduction;80
9.2;II. Weak Acids;82
9.3;III. Weak Bases;91
9.4;IV. Microelectrodes;94
9.5;V. Nuclear Magnetic Resonance Method;96
9.6;VI. Conclusions;99
9.7;Acknowledgments;102
9.8;References;103
10;Chapter 3. Mitochondrial ATPases;106
10.1;I. Introduction;107
10.2;II. Subunit Composition and Structure of F;108
10.3;III. Composition and Structure of the Intact ATPase Complex;113
10.4;IV. Chemical and Physical Properties of ATPase Subunits;116
10.5;V. Biosynthesis of ATPase;123
10.6;VI. Reactions Catalyzed by Isolated ATPase and Its Component Subunits;125
10.7;VII. Kinetics of ATPase Reactions;140
10.8;VIII. Relationship of Uncouplers to the Mode of Action of ATPase;143
10.9;IX. Ion Translocation by Mitochondrial ATPase;145
10.10;X. Linking Ion Translocation to the Chemical Formation of ATP;149
10.11;XI. Electrochemical Potential-Driven Synthesis of ATP;153
10.12;XII. Questions Regarding the in Vivo Mode of ATP Synthesis;154
10.13;References;156
11;Chapter 4. Ionophores and Ion Transport Across Natural Membranes;164
11.1;I. Definitions;164
11.2;II. Introduction;165
11.3;III. Methods for Measuring Ionophoric Activity;168
11.4;IV. Natural Membrane Ion Transport Systems Containing Ionophores;171
11.5;V. General Properties of Ion Transport Systems;188
11.6;VI. Summary and Concluding Remarks;189
11.7;Acknowledgments;190
11.8;References;190
12;Chapter 5. Reaction Mechanisms for ATP Hydrolysis and Synthesis in the Sarcoplasmic Reticulum;196
12.1;I. Introduction;197
12.2;II. Structure of the Sarcoplasmic Reticulium (SR) Membrane;199
12.3;III. Properties of ATP Hydrolysis by SR in the Steady State;203
12.4;IV. EP Formation and P¡ Liberation in the Presteady State;208
12.5;V. Reaction Mechanisms of ATP and p-Nitrophenyl Phosphate Hydrolysis by SR;216
12.6;VI. Kinetic Analysis of the Couphng of Ca2+ Transport with ATP Hydrolysis;227
12.7;VII. Molecular Models for Ca2+ Transport;234
12.8;VIII. Reaction Mechanism of ATP Synthesis;239
12.9;IX. Concluding Remarks;247
12.10;Acknowledgments;249
12.11;References;249
13;Chapter 6. Flavoproteins, Iron Proteins, and Hemoproteins as Electron-Transfer Components of the Sulfate-Reducing Bacteria;254
13.1;I. Introduction;255
13.2;II. Flavodoxin;256
13.3;III. Ferredoxin;258
13.4;IV. Cytochromes;260
13.5;V. Molybdenum-Containing Iron-Sulfur Protein from Desulfovibrio gigas;265
13.6;VI. Rubredoxin-Type Proteins;266
13.7;VII. Hydrogenase;268
13.8;VIII. Adenylyl Sulfate Reductase (APS Reductase);269
13.9;IX. Siroheme-Type Enzymes (Bisulfite and Sulfite Reductases);271
13.10;X. Bioenergetics of Respiratory Sulfate Reduction;275
13.11;XI. Conclusion;277
13.12;Acknowledgments;279
13.13;References;279
14;Chapter 7. Applications of the Photoaffinity Technique to the Study of Active Sites for Energy Transduction;284
14.1;I. Introduction;285
14.2;II. Photoaffinity Analogs of Ligands Used in Energy- Transduction Systems;316
14.3;III. Current Investigations on Specific Energy-Transducing Systems;330
14.4;IV. Concluding Comments;420
14.5;Acknowledgments;422
14.6;References;423
15;Subject Index;432