E-Book, Englisch, Band 140, 212 Seiten, eBook
Reihe: Methods in Molecular Biology
Schneider Chaperonin Protocols
Erscheinungsjahr 2008
ISBN: 978-1-59259-061-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, Band 140, 212 Seiten, eBook
Reihe: Methods in Molecular Biology
ISBN: 978-1-59259-061-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
Purification of Archaeal Chaperonin from Sulfolobus shibatae.- Purification of Hsp60 from Thermus thermophilus.- Purification of GroEL from an Overproducing E. coliStrain.- Purification of GroES from an Overproducing E. coliStrain.- Purification of the Gp31 Co-chaperonin of BacteriophageT4.- Removing Trace Fluorescent Contaminants from GroEL Preparations.- Assembly and Disassembly of GroEL and GroES Complexes.- GroEL/GroES Interaction Assayed by Protease Protection.- Determination of Chaperonin Activity In Vivo.- Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL.- Prevention of Rhodanese Aggregation by the Chaperonin GroEL.- Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES.- Assay of Malate Dehydrogenase.- Assay of Chaperonin-Assisted Refolding of Citrate Synthase.- Purification of Yeast Mitochondrial Hsp60.- Preparation of Recombinant Human Hsp10.- Purification of the Cytosolic ChaperoninTRiC from Bovine Testis.- Monitoring Actin Folding.- Folding Assays.- Purification of Prefoldin.- Purification of GimC from Saccharomyces cerevisiae.- Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding.
