Starkey / Elaswarapu Genomics

Essential Methods
1. Auflage 2010
ISBN: 978-0-470-71162-0
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 360 Seiten, E-Book

Reihe: Essential Methods

Genomics research has made significant advances in recent years. Inthis book, a team of internationally-renowned researchers share themost up-to-date information in a field that has in recent yearsswitched emphasis from gene identification to functional genomicsand the characterization of genes and gene products. This volumeapproaches this complex subject with a broad perspective to supplythe reader with a vital overview of genomics and its derivativefields, with a focus on pivotal issues such as data analysis.Expansive and current, this book is a comprehensiveresearch guide that describes both the key new techniques and moreestablished methods. Every chapter discusses the merits andlimitations of the various approaches and then provides selectedtried-and-tested protocols, as well as a plethora of good practicaladvice for immediate use at the bench.
Key features:
* Provides a broad introduction to current practices andtechniques for lab-based research in genomics
* Explains clearly and precisely how to carry out selectedtechniques in addition to background information on the variousapproaches
* Chapters are written by a leading international authorities inthe field and cover both well-known and new, tried and tested,methods for working in genomics
* Includes troubleshooting guide and reviews of alternativetechniques
* An essential laboratory manual for students and researchers atall levels

Autoren/Hrsg.

Starkey, Mike

Elaswarapu, Ramnath

Inhaltsverzeichnis

List of Contributors.
Preface.
1 High-Resolution Analysis of Genomic Copy Number Changes(Mario Hermsen, Jordy Coffa, Bauke Ylstra, Gerrit Meijer, HansMorreau, Ronald van Eijk, Jan Oosting and Tom van Wezel).
1.1 Introduction.
1.2 Methods and Approaches.
1.3 Troubleshooting.
References.
2 Identification of Polymorphic Markers for GeneticMapping (Daniel C. Koboldt and Raymond D. Miller).
2.1 Introduction.
2.2 Methods and Approaches.
2.3 Troubleshooting.
References.
3 Genotyping and LOH Analysis on Archival Tissue Using SNPArrays (Ronald van Eijk, Anneke Middeldorp, Esther H. Lips,Marjo van Puijenbroek, Hans Morreau, Jan Oosting and Tom vanWezel).
3.1 Introduction.
3.2 Methods and Approaches.
3.3 Troubleshooting.
References.
4 Genetic Mapping of Complex Traits (Nancy L.Saccone).
4.1 Introduction.
4.2 Methods and Approaches.
4.3 Troubleshooting.
References.
5 RNA Amplification Strategies: Toward Single-CellSensitivity (Natalie Stickle, Norman N. Iscove, CarlVirtanen, Mary Barbara, Carolyn Modi, Ari Baratz, Toni DiBerardino, Ellen Greenblatt, Ted Brown and NeilWinegarden).
5.1 Introduction.
5.2 Methods and Approaches.
5.3 Troubleshooting.
References.
6 Real-Time Quantitative RT-PCR for mRNA Profiling(Stephen A. Bustin and Tania Nolan).
6.1 Introduction.
6.2 Methods and Approaches.
6.3 Troubleshooting.
References.
7 Gene Expression in Mammalian Cells (FélixRecillas-Targa, Georgina Guerrero, Martín Escamilla-del-Arenaland Héctor Rincón-Arano).
7.1 Introduction.
7.2 Methods and Approaches.
7.3 Troubleshooting.
Acknowledgments.
References.
8 Using Yeast Two-Hybrid Methods to Investigate Large Numbersof Binary Protein Interactions (Panagoula Charalabous,Jonathan Woodsmith and Christopher M. Sanderson).
8.1 Introduction.
8.2 Methods and Approaches.
8.3 Troubleshooting.
References.
9 Prediction of Protein Function (Hon NianChua).
9.1 Introduction.
9.2 Methods and Approaches.
9.3 Troubleshooting.
References.
10 Elucidating Gene Function through Use of GeneticallyEngineered Mice (Mary P. Heyer, Cátia Feliciano,João Peca and Guoping Feng).
10.1 Introduction.
10.2 Methods and Approaches.
10.3 Troubleshooting.
References.
11 Delivery Systems for Gene Transfer (CharlotteLawson and Louise Collins).
11.1 Introduction.
11.2 Methods and Approaches.
11.3 Troubleshooting.
References.
12 Gene Therapy Strategies: Constructing an AAV TrojanHorse (M. Ian Phillips, Edilamar M. de Oliveira, LepingShen, Yao Liang Tang, and Keping Qian).
12.1 Introduction.
12.2 Methods and Approaches.
12.3 Troubleshooting.
References.
13 An Introduction to Proteomics Technologies for theGenomics Scientist (David B. Friedman).
13.1 Introduction.
13.2 Methods and Approaches.
13.3 Troubleshooting.
References.
Index.