Thorner Applications of Chimeric Genes and Hybrid Proteins, Part B: Cell Biology and Physiology

1. Auflage 2000
ISBN: 978-0-08-049682-5
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 712 Seiten, Web PDF

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Autoren/Hrsg.

Thorner, Jeremy

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 327;12
4;Preface;18
5;Volumes in Series;20
6;Section I: Epitope Tags for Immunodetection;39
6.1;Chapter 1. Epitope Tagging: General Method for Tracking Recombinant Proteins;41
7;Section II: Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing;55
7.1;Chapter 2. Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Cells, Tissues, and Embryos;57
7.2;Chapter 3. Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Localization in the Electron Microscope;73
7.3;Chapter 4. Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras;83
7.4;Chapter 5. Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein;99
7.5;Chapter 6. Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stomatitis Virus Protein G–Green Fluorescent Protein Hybrids;107
7.6;Chapter 7. Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Conventional Fluorescence Microscopy and 35-mm Photography;127
7.7;Chapter 8. Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast;133
7.8;Chapter 9. Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Transport in Yeast Secretory Pathway;145
7.9;Chapter 10. Lineage Analysis with Retroviral Vectors;156
7.10;Chapter 11. Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags;183
8;Section III: Tools for Analysis of Membrane Proteins;201
8.1;Chapter 12. A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo;203
8.2;Chapter 13. Using SUC2–HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyces cerevisiae;213
8.3;Chapter 14. Detecting Interactions between Membrane Proteins in Vivo Using Chimeras;228
8.4;Chapter 15. Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Receptors and Their Ligands;236
8.5;Chapter 16. Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation;248
8.6;Chapter 17. Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms;266
8.7;Chapter 18. Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Receptors of Eukaryotic Cells;277
8.8;Chapter 19. Green Fluorescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring Ion Channel Function;287
8.9;Chapter 20. Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis;298
9;Section IV: Signals for Addressing Proteins to Specific Subcellular Compartments;315
9.1;Chapter 21. Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum;317
9.2;Chapter 22. Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags;321
9.3;Chapter 23. Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae;335
9.4;Chapter 24. Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals;343
9.5;Chapter 25. Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoylation and Palmitoylation;355
9.6;Chapter 26. Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use of Lipid-Modified Plasma Membrane-Targeting Sequences;369
9.7;Chapter 27. Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins;389
9.8;Chapter 28. Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocation and Nonclassic Exocytosis;407
10;Section V: Application of Chimeras in Monitoring and Manipulating Cell Physiology;421
10.1;Chapter 29. Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains;423
10.2;Chapter 30. Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles and Advances;439
10.3;Chapter 31. Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins;459
10.4;Chapter 32. Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificial Dimerization Domains to Activate Tyrosine Kinases;467
10.5;Chapter 33. Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compartments;478
10.6;Chapter 34. Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells;494
10.7;Chapter 35. Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein;510
10.8;Chapter 36. Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein;538
10.9;Chapter 37. Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis;551
10.10;Chapter 38. Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein and Synaptic Vesicle Membrane Proteins as Reporters of Neurotransmitter Release;560
10.11;Chapter 39. Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin Conjugates;584
10.12;Chapter 40. Fluorescent Labeling of Recombinant Proteins in Living Cells with FlAsH;603
10.13;Chapter 41. Ubiquitin Fusion Technique and Its Descendants;616
10.14;Chapter 42. Use of Phosphorylation Site Tags in Proteins;632
11;Author Index;653
12;Subject Index;695