Tsao Annual Reports on Fermentation Processes

1. Auflage 2014
ISBN: 978-1-4832-1386-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 8, 336 Seiten, Web PDF

Reihe: Annual Reports on Fermentation Processes

Annual Reports on Fermentation Processes, Volume 8 emphasizes the significant developments in fermentation processes. This book discusses the formation of protosomes with yeast proteins, kinetics of the inhibition of yeast growth and fermentation by ethanol, and bioseparation by cartridge chromatography. The concentration polarization and shear deactivation of protein during ultrafiltration/diafiltration and chromatographic purification of semisynthetic beta-lactam antibiotics are also elaborated. This publication likewise covers the regulation of cellulolytic activity, computer aids for teaching biotechnology, and steroid bioconversions. Other topics include the cellulose pretreatments and modeling of adsorption kinetics of cellulases in cellulose column. This volume is useful to students and researchers conducting work on fermentation research and developments.

Autoren/Hrsg.

Tsao, George T.

Inhaltsverzeichnis

1;Front Cover;1
2;Annual Reports on Fermentation Processes;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors;8
6;Preface;10
7;Chapter 1. The Protosome: A Useful Artifact for Protein Purification;12
7.1;ABSTRACT;12
7.2;INTRODUCTION;13
7.3;THE FORMATION OF PROTOSOMES IN PLANT SYSTEMS;14
7.4;FORMATION OF PROTOSOMES WITH YEAST PROTEINS;14
7.5;FUTURE WORK ON MICROBIAL (YEAST) PROTOSOMES;20
7.6;CONCLUSIONS;21
7.7;REFERENCES;21
8;Chapter 2. Ethanol Toxicity and Ethanol Tolerance in Yeasts;22
8.1;I. INTRODUCTION;22
8.2;II. KINETICS OF THE INHIBITION OF YEAST GROWTH AND FERMENTATION BY ETHANOL;23
8.3;III. EFFECTS OF ETHANOL AND OTHER ALKANOLS ON THE TEMPERATURE RELATIONS OF S. CEREVISIAE AND OTHER YEASTS;29
8.4;IV. EFFECTS OF ETHANOL AND OTHER ALKANOLS ON THE MEMBRANE TRANSPORT OF NUTRIENTS IN S. CEREVISIAE AND OTHER YEASTS;39
8.5;V. EFFECTS OF ETHANOL ON GLYCOLYTIC ENZYMES;49
8.6;VI. THE QUESTION OF THE INTRACELLULAR ACCUMULATION OF ETHANOL;51
8.7;VII. INTERACTION OF ETHANOL AND OTHER ALKANOLS WITH MEMBRANES;54
8.8;VIII. CONCLUSIONS;60
8.9;REFERENCES;62
9;Chapter 3. Bioseparation by Cartridge Chromatography;70
9.1;INTRODUCTION;70
9.2;RESULT AND DISCUSSION;71
9.3;REFERENCES;82
10;Chapter 4. Concentration Polarization and Shear Deactivation of Protein during Ultrafiltration/Diafiltration;84
10.1;ABSTRACT;84
10.2;INTRODUCTION;85
10.3;MATERIALS AND METHODS;86
10.4;MEMBRANE APPARATUS AND PROCEDURE;88
10.5;RESULTS AND DISCUSSION;90
10.6;CONCLUSION;101
10.7;ACKNOWLEDGMENT;101
10.8;REFERENCES;101
11;Chapter 5. Chromatographie Purification of Semisynthetic Beta-Lactam Antibiotics;104
11.1;ABSTRACT;104
11.2;INTRODUCTION;104
11.3;SYNTHESIS;105
11.4;ANALYTICAL HPLC;105
11.5;PREPARATIVE CHROMATOGRAPHY;111
11.6;PROCESS SCALE CHROMATOGRAPHY;113
11.7;OTHER ANTIBIOTICS;120
11.8;USER EXPERIENCE WITH PROCESS HPLC;120
11.9;CONCLUSION;120
11.10;REFERENCES;121
12;Chapter 6. Regulation of Cellulolytic Activity;122
12.1;I. INTRODUCTION;122
12.2;II. REGULATION OF ACTIVITY AT THE BIOSYNTHETIC LEVEL;123
12.3;III. REGULATION OF ACTIVITY AT THE SECRETORY LEVEL;148
12.4;IV. REGULATION AT THE CATALYTIC LEVEL;153
12.5;ACKNOWLEDGMENTS;158
12.6;REFERENCES;158
13;Chapter 7. Computer Aids for Teaching Biotechnology;166
13.1;LANGUAGES;166
13.2;GAMES AND SIMULATION EXERCISES;167
13.3;TUTORIALS;169
13.4;EXAMPLES;172
13.5;DISCUSSION;180
13.6;ACKNOWLEDGMENT;180
13.7;REFERENCES;180
13.8;ADDITIONAL READING;180
14;Chapter 8. Steroid Bioconversions: One Industrial Perspective;182
15;Chapter 9. Solubilizing Cellulosic Materials by Alkali-Cooking for Anaerobic Methane Production;198
15.1;ABSTRACT;198
15.2;BACKGROUND;200
15.3;EXPERIMENTAL;204
15.4;RESULTS AND DISCUSSION;206
15.5;CONCLUSIONS;218
15.6;REFERENCES;218
16;Chapter 10. Surface Adsorption and Reaction Kinetics of Enzymatic Cellulose Hydrolysis in a Column Reactor;222
16.1;1. INTRODUCTION;222
16.2;2. A REVIEW AND ANALYSIS OF ENZYME KINETICS;224
16.3;3. A REVIEW OF ADSORPTION THEORY AND ANALYSIS TECHNIQUES;244
16.4;4. MODELING OF ADSORPTION KINETICS OF CELLULASES IN CELLULOSE COLUMN;250
16.5;5. EXPERIMENTAL METHODS;264
16.6;6. MODEL STUDY OF CELLULASE ADSORPTION AND KINETICS IN COLUMNS OF CELLULOSE BEADS;300
16.7;7. CONCLUDING REMARKS;306
16.8;8. REFERENCES;306
17;Chapter 11. Cellulose Pretreatments: Technology and Techniques;310
17.1;ABSTRACT;310
17.2;BACKGROUND;311
17.3;COMMENTS ON THE DATA;313
17.4;CONCLUSIONS;330
17.5;RECOMMENDATIONS;331
17.6;REFERENCES;332
18;Index;336