Vanden Heuvel PCR Protocols in Molecular Toxicology

Erscheinungsjahr 1997
ISBN: 978-1-4398-0580-0
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 240 Seiten

Reihe: Methods in Life Sciences - Toxicology Section

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

- Quantitative reverse transcriptase PCR and methods to examine gene expression

- Differential display cloning

- Cloning and library screening by PCR

- Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes

- Basic, non-PCR based molecular biology methods

PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Autoren/Hrsg.

Vanden Heuvel, John P.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Toxikologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Basics of the Polymerase Chain Reaction, W.B. Mattes
Analysis of Gene Expression, J.P. Vanden Heuvel
Differential Display PCR, J.C. Corton
Cloning by PCR, J.P. Vanden Heuvel
Genotype Analysis, D.A. Bell and G. Pittman
General Molecular Biology Techniques, J.P. Vanden Heuvel
Appendix I: List of Suppliers
Appendix II: Companies, Online Catalogs, and Internet Resources
Index