Buch, Englisch, Band 379, 288 Seiten, Paperback, Format (B × H): 170 mm x 244 mm, Gewicht: 542 g
Practical Protein Engineering
Buch, Englisch, Band 379, 288 Seiten, Paperback, Format (B × H): 170 mm x 244 mm, Gewicht: 542 g
Reihe: Advances in Experimental Medicine and Biology
ISBN: 978-1-4613-7995-9
Verlag: Springer US
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
I. Structural Studies of Subtilisin Enzymes Alone and in Complex with Inhibitors.- 1. The Complex between Mesentericopeptidase and Eglin-C.- 2. Crystallographic Study of Eglin-C Binding to Thermitase.- 3. Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using ?, ß-Dehydro-Residues.- 4. Structure and Function of Subtilisin BPN’ as Studied through Crystallographic Studies on a Series of Its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI.- 5. Three Dimensional Structure of the Antibiotic Bacitracin A Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition.- 6. Active Site Binding Loop Stabilization in the Subtilisin Inhibitor Eglin C: Structural and Functional Studies on Specifically Designed Mutants in Complex with Subtilisin and the Uncomplexed Inhibitor.- 7. Crystal Strucutures of the Alkaline Proteases Savinase and Esperase from Bacillus lentus.- II. New Enzymes.- 8. Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-like Enzymes of Unknown 3-Dimensional Structure.- 9. Substilases: Subtilisin-like Serine Proteases.- 10. Cloning and Expression of the Gene Encoding a Novel Proteinase from Tritirachium album Limber.- III. Structure-Function Studies.- 11. Studies of Binding Sites in the Subtilisin from
Bacillus lentus by Means of Site Directed Mutagenesis and Kinetic Investigations.- 12. Laundry Performance of Subtilisin Proteases.- 13. Free Energy Perturbation Techniques Applied to Subtilisin BPN’ Stability.- IV. Biophysical Probes and Mutagenesis.- 14. Thermitase-Kinetic Differentiation to the Subtilisins.- 15. Fluorescence Decay of Tryptophans in Serine Proteinases from Microorganisms: Relation to X-Ray Models.- 16. Propeptide-Mediated Folding in Subtilisin: The IntramolecularChaperone Concept.- 17. Roles of the N- and C-Terminal Pro-Sequences of Aqualysin I Precursor in the Processing and Extracellular Secretion of the Enzyme.- 18. Crystal Structure Analysis of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability.- 19. Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermostability by Temperature-Gradient Gel Electrophoresis.- 20. Inhibition of Proteinase K by Mercury.- V. Protein Engineering of Subtilisin Enzymes.- 21. Crystallographic Investigations of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability.- 22. Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92.- 23. Engineering Surface Charges in a Subtilisin.- 24. pH Dependence of the Catalytic Activity of a Subtilisin-like Proteinase.- 25. An Investigation of the Savinase Water Channel: Implications of Cavity Mutations.- 26. Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus lentus Alkaline Proteases.- 27. Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL.- 28. Studies on the Structure and Function of Subtilisin E by Protein Engineering.- 29. Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus lentus Subtilisin.