Metastasis Research Protocols

ISBN: 978-0-89603-615-4
Verlag: Springer, Berlin

Buch, Englisch, Band 58, 336 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1 g

Reihe: Methods in Molecular Medicine

Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe in detail the key methods needed to investigate why and how metastasis occurs. Volume II of this two-volume set, Analysis of Cell Behavior In Vitro and In Vivo, presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and in whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. The first volume of this set, Analysis of Cells and Tissues, focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression.
Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.

Autoren/Hrsg.

Brooks, Susan A.

Schumacher, Udo

Inhaltsverzeichnis

Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior
Cell Separations by Flow Cytometry
Derek Davies
Immunomagnetic Cell Separation
Catherine Clarke and Susan Davies
Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
Daniel McWilliams and Hilary Collins
Part II. In Vitro Assays of Metastatic Behavior
Cell Aggregation Assays, Tom Boterberg
Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel
Cell Migration and the Boyden Chamber
Nicholas S. Brown and Roy Bicknell
Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Yasufumi Niinaka, Arayo Haga, and Avraham Raz
In Vitro Invasion Assay Using Matrigel®
Debbie M. S. Hall and Susan A. Brooks
Membrane Invastion Culture System
Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor
Collagen Invasion Assay
Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel
Chick Heart Invasion Assay
Marc E. Bracke, Tom Boterberg, and Marc M. Mareel
Adhesion of Tumor Cells to Matrices and Endothelium
G. Ed Rainger
Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay
Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell
Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis
Nerbil Kilic and Süleyman Ergün
Galectin-3 Binding and Metastasis
Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz
Part III. Animal Models of Metastasis
Basic Principles for the Study of Metastasis Using Animal Models
Suzanne A. Eccles
The Chick Embryo Metastasis Model
Eric Petitclerc and Peter C. Brooks
Gene Transfection for Metastasis Research Using Animal Models
Ruth J. Muschel and Jim Hua
Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
Sue A. Watson and Teresa M. Morris
Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models
Janet E. Price
Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis
Udo Schumacher and Susan A. Brooks
Syngeneic Murine Metastasis Model: B16 Melanoma
Raffaella Giavazzi and Angela Garofalo
Transgenic Animal Models
Chantale Guy and Gizela Cordosa
Clinically Accurate Orthotopic Mouse Models of Cancer
Robert M. Hoffman
Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo
Marian Rocha, Volker Schirrmacher, and Victor Umansky
Green Fluorescent Protein for Metastastis Research
Robert M. Hoffman