Laboratory Manual and Workbook in Microbiology: Applications to Patient Care

10. Auflage 2010
ISBN: 978-0-07-352253-1
Verlag: McGraw Hill LLC

Buch, Englisch, 336 Seiten, Format (B × H): 224 mm x 269 mm, Gewicht: 703 g

The emphasis of this lab manual is on the basic principles of diagnostic microbiology for students preparing to enter an allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and to practice safety in the laboratory and hospital environment. It remains oriented primarily toward meeting the interests and needs of those who will be directly involved in patient care and who wish to learn how microbiological principles should be applied in the practice of their professions. The authors have emphasised the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. The exercises illustrate as simply as possible the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilisation. Attention is given to the role of the health professional in regard to appropriate collection of clinical specimens and the applications of aseptic and disinfectant techniques as they relate to patient care.

Autoren/Hrsg.

Granato, Paul A.

Morello, Josephine

Morton, Verna

Granato, Paul

Mizer, Helen Eckel

Mizer, Helen

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Labormedizin
• Naturwissenschaften Biowissenschaften Mikrobiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie

Inhaltsverzeichnis

PART 1 Basic Techniques of Microbiology1. The Microscope2. Handling and Examining Cultures3. Hanging Drop and Wet-Mount Preparations4. Simple Stains5. Gram Stain6. Acid-Fast Stain7. Special Stains8. Culture Media9. Streaking Technique to Obtain Pure Cultures10. Pour-Plate and Subculture Techniques11. Culturing Microorganisms from the EnvironmentPART 2 Destruction of Microorganisms12. Moist and Dry Heat13. The Autoclave14. Disinfectants15. Antimicrobial Agent Susceptibility Testing and ResistancePART 3 Diagnostic Microbiology in Action16. Primary Media for Isolation of Microorganisms17. Some Metabolic Activities of Bacteria18. Activities of Bacterial Enzymes19. Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms.20. Staphylococci21. Streptococci, Pneumococci, and Enterococci22. Haemophilus, Corynebacteria, and Bordetella23. Clinical Specimens from the Respiratory Tract24. The Enterobacteriaceae (Enteric Bacilli)25. Clinical Specimens from the Intestinal Tract26. Urine Culture Techniques27. Neisseria and SpirochetesPART 4Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients’ Antibodies28. Anaerobic Bacteria29. Mycobacteria30. Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions31. Fungi: Yeast and Molds32. Protozoa and Animal Parasites33. Serodiagnosis of Infectious DiseasePART 5 Applied (Sanitary) Microbiology34. Bacteriological Analysis of Water35. Bacteriological Analysis of Milk