Buch, Englisch, Band 2400, 320 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 831 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2400, 320 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 831 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-1834-9
Verlag: Springer US
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Preface…
Table of Contents…
Contributing Authors…
1 In Vivo Detection of Double-Stranded RNA by dRBFC Assay
Xiaofei Cheng, Yameng Luan, and Aiming Wang
2 Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology
Keyan Xu and Yi Li
3 Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions
Yu Huang and Yi Li
4 Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors
Fangfang Li and Aiming Wang
5 Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research
Zhaoji Dai and Aiming Wang
6 Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions
Yan Liang and Zhenghe Li
7 Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants
Zhaoji Dai and Aiming Wang
8 Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay
Ziwei Tang, Mark A. Bernards, and Aiming Wang
9 A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay
Zhihao Jiang, Meng Yang, Qing Cao, Yongliang Zhang, and Dawei Li
10 Membrane and Nuclear Yeast Two-Hybrid Systems
Qian Chen and Taiyun Wei
11 Tag-Based Pull-Down Assay
Shanwu Lyu, Changwei Zhang, Xilin Hou, and Aiming Wang
12 Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses
Rongrong He, Mark A. Bernards, and Aiming Wang
13 Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants
Aaron Simkowich, Susanne E. Kohalmi, and Aiming Wang
14 Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity
Shanshan Zhao and Jianguo Wu
15 Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors
Tao Zhou, Laihua Dong, Tong Jiang, and Zaifeng Fan
16 A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus
Ji-Hui Qiao, Qiang Gao, Ying Zang, Xiao-Dong Fang, and Xian-Bing Wang
17 Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques
Keru Yan, Xuejie Du, and Bizeng Mao
18 Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips
Min-Rui Wang, Tianxing Pang, Ziqian Lian, Qiao-Chun Wang, and Liying Sun
19 Establishment of White-Backed Planthopper Cell Lines
Hongyan Chen, Wei Wu, and Taiyun Wei
20 Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses
Yinzi Li and Aiming Wang
21 Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues
Yuxin Ma and Shifang Li
22 Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses
Ali Raza and Qingfa Wu
23 Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses
Subhas Hajeri and Raymond Yokomi
24 Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA
Shibo Gao and Jianxiang Wu
25 Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees
Liping Wang
26 Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR
Yi Xie and Jianxiang Wu
27 RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants
Jing-Ze Zou, De-Shui Liu, Xin Tong, Xiao-Peng Zhang, and Xian-Bing Wang
28 Transmission Electron Microscopic Methods for Plant Virology
Li Xie and Jian Hong
Subject Index List…
