Buch, Englisch, 330 Seiten, Format (B × H): 262 mm x 180 mm, Gewicht: 858 g
Fundamental Mechanisms and Methodologies
Buch, Englisch, 330 Seiten, Format (B × H): 262 mm x 180 mm, Gewicht: 858 g
ISBN: 978-0-367-53815-6
Verlag: Taylor & Francis Ltd
Volume I, Part 1 covers fundamental topics such as the basic principles of ion permeation and selectivity, voltage-dependent, ligand-dependent, and mechano-dependent ion channel activation mechanisms, the mechanisms for ion channel desensitization and inactivation, and basic ion channel pharmacology and inhibition. Volume I, Part 2 offers a practical guide of cardinal methods for researching ion channels, including heterologous expression and voltage-clamp and patch-clamp electrophysiology; isolation of native currents using patch clamping; modeling ion channel gating, structures, and its dynamics; crystallography and cryo-electron microscopy; fluorescence and paramagnetic resonance spectroscopy methods; and genetics approaches in model organisms.
All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels; a guide to the technical aspects of ion channel research; a modern guide to the properties of major ion channel families; and includes coverage of key examples of regulatory, physiological and disease roles for ion channels.
Zielgruppe
Postgraduate and Undergraduate Advanced
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Physik Physik Allgemein
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie
- Interdisziplinäres Wissenschaften Wissenschaften Interdisziplinär Neurowissenschaften, Kognitionswissenschaft
Weitere Infos & Material
Part I: Fundamental Mechanisms 1. Ion Selectivity and Conductance 2. Voltage-Dependent Gating of Ion Channels 3. Ligand-Dependent Gating Mechanism 4. Mechanosensitive Channels and Their Emerging Gating Mechanisms 5. Inactivation and Desensitization 6. Ion Channel Inhibitors Part II: Methodologies 7. Expression of Channels in Heterologous Systems and Voltage Clamp Recordings of Macroscopic Currents 8. Patch Clamping and Single-Channel Analysis 9. Patch Clamp Recordings from Native Cells and Isolation of Membrane Currents 10. Models of Ion Channel Gating 11. Investigating Ion Channel Structure and Dynamics Using Fluorescence Spectroscopy 12. Ion Channel Structural Biology in the Era of Single Particle Cryo-EM 13. Protein Crystallography 14. Rosetta Structural Modeling 15. Molecular Dynamics 16. Genetic Models and Transgenics 17. EPR and DEER Spectroscopy