Ludtke Recent Advances in Electron Cryomicroscopy, Part B

1. Auflage 2011
ISBN: 978-0-12-386508-3
Verlag: Elsevier Book Series
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 144 Seiten

Electron cryomicroscopy is a form of transmission electron microscopy (EM) in which the sample is studied at cryogenic temperatures (generally liquid nitrogen temperatures). Cryo-EM is developing popularity in structural biology. This volume from the Advances in Protein Chemistry and Structural Biology series is Part B and covers essential topics.

Autoren/Hrsg.

Ludtke, Steve

Inhaltsverzeichnis

1;Front Cover;1
2;Recent Advances in Electron Cryomicroscopy, Part B;4
3;Copyright;5
4;Contents;6
5;Chapter 1: Atomic resolution cryo electron microscopy of macromolecular complexes;10
5.1;I. Introduction;11
5.2;II. Atomic Structures of Viruses at Stable and Metastable States by CryoEM;12
5.3;III. Structures of Molecular Assemblies of Low-Symmetry and Asymmetry;14
5.4;IV. Resolving Structures Not Seen by Conventional Methods of Atomic Structure Determination;19
5.5;V. Strategies for Atomic Structure Determination by CryoEM;23
5.6;VI. Conclusions and Future Perspective;37
5.7;Acknowledgments;38
5.8;References;38
6;Chapter 2: Single-particle electron cryotomography (cryoET);46
6.1;I. Introduction;47
6.2;II. Description of Methods;49
6.3;III. Applications of Single-Particle Tomography;57
6.4;IV. Practical Considerations for Alignment Searches;65
6.5;V. Future Developments;68
6.6;Acknowledgments;68
6.7;References;69
7;Chapter 3: Cellular tomography;76
7.1;I. Introduction;77
7.2;II. Cellular Tomography on Rapidly Frozen/Freeze-Substituted Specimens;81
7.3;III. Tomographic Approaches for Large Volume Reconstructions;87
7.4;IV. Cellular Cryo-Electron Tomography on Vitrified Intact Small Cells and Flattened Areas of Surface-Adsorbed Cells...;88
7.5;V. Cellular Cryo-Electron Tomography on Vitrified Sections of Cells and Tissues;90
7.6;VI. Conclusions;92
7.7;Acknowledgments;95
7.8;References;95
8;Chapter 4: Correlative light-electron microscopy;100
8.1;I. Introduction;100
8.2;II. Correlative Light and Electron Microscopy (cLEM) Implementation;101
8.3;III. Future Perspectives;103
8.4;IV. Outlook;104
8.5;Acknowledgments;106
8.6;References;106
9;Chapter 5: Future prospects;110
9.1;I. Introduction: History of Structural Biology and the Increasing Importance of Electron Microscopy;111
9.2;II. The Beginnings of Cryo-Electron Tomography;116
9.3;III. Successes in Cryo-Electron Tomography;117
9.4;IV. Radiation Damage and the Missing Wedge;118
9.5;V. High-Resolution Cryo-EM Reconstructions;120
9.6;VI. Molecular Models Derived from Cryo-EM Reconstructions;121
9.7;VII. Development of Validation Criteria;122
9.8;VIII. Data Deposition Policy;123
9.9;IX. Extrapolate into the Future;124
9.10;Acknowledgments;125
9.11;References;125
10;Author Index;132
11;Subject Index;142
12;Colour Plate;146